@article{c835c5a1fd33417fac0f1f537016b958,
title = "Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens",
abstract = "Mechanisms of infection and pathogenesis have predominantly been studied based on differential gene or protein expression. Less is known about posttranslational modifications, which are essential for protein functional diversity. We applied an innovative glycoproteomics method to study the systemic proteome-wide glycosylation in response to infection. The protein site-specific glycosylation was characterized in plasma derived from well-defined controls and patients. We found 3862 unique features, of which we identified 463 distinct intact glycopeptides, that could be mapped to more than 30 different proteins. Statistical analyses were used to derive a glycopeptide signature that enabled significant differentiation between patients with a bacterial or viral infection. Furthermore, supported by a machine learning algorithm, we demonstrated the ability to identify the causative pathogens based on the distinctive host blood plasma glycopeptide signatures. These results illustrate that glycoproteomics holds enormous potential as an innovative approach to improve the interpretation of relevant biological changes in response to infection.",
keywords = "Glycobiology, Glycomics, Health sciences, Immunology",
author = "Esther Willems and Jolein Gloerich and Anouk Suppers and {van der Flier}, Michiel and {van den Heuvel}, {Lambert P.} and {van de Kar}, Nicole and Philipsen, {Ria H.L.A.} and {van Dael}, Maurice and Myrsini Kaforou and Wright, {Victoria J.} and Herberg, {Jethro A.} and Torres, {Federico Martinon} and Michael Levin and {de Groot}, Ronald and {van Gool}, {Alain J.} and Lefeber, {Dirk J.} and Wessels, {Hans J.C.T.} and {de Jonge}, {Marien I.} and {PERFORM consortium} and Dace Zavadska and Anda Balode and Arta Bārzdiņa and Dace Gardovska and Dagne Grāvele and Ilze Grope and Anija Meiere and Ieva Nokalna and Urbāne, {Urzula Nora} and Jana Pavāre and Zanda Pu{\v c}uka and Dārta Deksne and Katrina Selecka and Aleksandra Sidorova",
note = "Funding Information: This research, part of the PERFORM project, has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under grant agreement No. 668303 . The samples collected previously were funded by: the European Seventh Framework Programme for Research and Technological Development ( FP7 ) under EUCLIDS Grant Agreement No. 279185 . Funding Information: This research, part of the PERFORM project, has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No. 668303. The samples collected previously were funded by: the European Seventh Framework Programme for Research and Technological Development (FP7) under EUCLIDS Grant Agreement No. 279185. We would like to thank all the patients and the healthy controls for donating their blood for the EUCLIDS, IRIS, and Westra studies. We would like to thank the Radboud Consortium for Glycoscience for their advice for data interpretation. We also thank the PERFORM consortium (see supplemental material for all participants) for their collaboration and fruitful discussions. Conceptualization, E.W. H.W. and M.J.; Methodology, E.W. M.D. H.W. and M.J.; Investigation, E.W.; Software, A.S. and H.W.; Formal Analysis, E.W. and H.W.; Visualization, E.W. and H.W.; Resources, M.F. L.H. N.K. R.P. M.K. V.W. J.H. and F.M.; Writing – Original Draft, E.W. and M.J.; Writing – Review & Editing, E.W. J.G. A.S. M.F. L.H. N.K. R.P. M.D. M.K. V.W. J.H. F.M. M.L. R.G. A.G. D.L. H.W. and M.J.; Funding Acquisition, M.J. R.G. and M.L.; Supervision, H.W. D.L. and M.J. The authors declare no competing interests. We support inclusive, diverse, and equitable conduct of research. Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = aug,
day = "18",
doi = "10.1016/j.isci.2023.107257",
language = "English",
volume = "26",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "8",
}