Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing

M. Dambrova, L. Baumane, I. Kalvinsh, J. E.S. Wikberg

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO) is frequently used as a spin trap for the measurement of superoxide by EPR spectrometry. However, its half life is fairly short in room temperature. We here show that superoxide radicals trapped by DEPMPO can be successfully recorded at -196°C. Moreover, we show that the signal intensity remains unaltered for up to 7 days, when the samples are stored in liquid nitrogen. Our new approach for measurement of superoxide should greatly simplify the studies of this important radical in biological systems. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)895-898
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume275
Issue number3
DOIs
Publication statusPublished - 7 Sep 2000
Externally publishedYes

Keywords

  • Allopurinol
  • DEPMPO
  • EPR
  • Liquid nitrogene
  • Superoxide
  • Superoxide dismutase
  • Xanthine oxidase

Field of Science

  • 3.1 Basic medicine
  • 1.4 Chemical sciences
  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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