Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing

M. Dambrova; L. Baumane; I. Kalvinsh; J. E.S. Wikberg

doi:10.1006/bbrc.2000.3387

Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing

M. Dambrova^*
, L. Baumane
, I. Kalvinsh
, J. E.S. Wikberg

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO) is frequently used as a spin trap for the measurement of superoxide by EPR spectrometry. However, its half life is fairly short in room temperature. We here show that superoxide radicals trapped by DEPMPO can be successfully recorded at -196°C. Moreover, we show that the signal intensity remains unaltered for up to 7 days, when the samples are stored in liquid nitrogen. Our new approach for measurement of superoxide should greatly simplify the studies of this important radical in biological systems. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	895-898
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	275
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.2000.3387
Publication status	Published - 7 Sept 2000
Externally published	Yes

Keywords*

Allopurinol
DEPMPO
EPR
Liquid nitrogene
Superoxide
Superoxide dismutase
Xanthine oxidase

Field of Science*

1.6 Biological sciences
3.1 Basic medicine
1.4 Chemical sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1006/bbrc.2000.3387

Cite this

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title = "Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing",

abstract = "5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO) is frequently used as a spin trap for the measurement of superoxide by EPR spectrometry. However, its half life is fairly short in room temperature. We here show that superoxide radicals trapped by DEPMPO can be successfully recorded at -196°C. Moreover, we show that the signal intensity remains unaltered for up to 7 days, when the samples are stored in liquid nitrogen. Our new approach for measurement of superoxide should greatly simplify the studies of this important radical in biological systems. (C) 2000 Academic Press.",

keywords = "Allopurinol, DEPMPO, EPR, Liquid nitrogene, Superoxide, Superoxide dismutase, Xanthine oxidase",

author = "M. Dambrova and L. Baumane and I. Kalvinsh and Wikberg, \{J. E.S.\}",

note = "Funding Information: Support was obtained from the Swedish MRC (04X-05957), the Swedish Heart and Lung Foundation (199941304), the {\AA}ke Wiberg Foundation, and Melacure Therapeutics. Dr. Maija Dambrova was supported by a stipend from the Novartis Foundation for Medical and Biological Research.",

year = "2000",

month = sep,

day = "7",

doi = "10.1006/bbrc.2000.3387",

language = "English",

volume = "275",

pages = "895--898",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

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TY - JOUR

T1 - Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing

AU - Dambrova, M.

AU - Baumane, L.

AU - Kalvinsh, I.

AU - Wikberg, J. E.S.

N1 - Funding Information: Support was obtained from the Swedish MRC (04X-05957), the Swedish Heart and Lung Foundation (199941304), the Åke Wiberg Foundation, and Melacure Therapeutics. Dr. Maija Dambrova was supported by a stipend from the Novartis Foundation for Medical and Biological Research.

PY - 2000/9/7

Y1 - 2000/9/7

N2 - 5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO) is frequently used as a spin trap for the measurement of superoxide by EPR spectrometry. However, its half life is fairly short in room temperature. We here show that superoxide radicals trapped by DEPMPO can be successfully recorded at -196°C. Moreover, we show that the signal intensity remains unaltered for up to 7 days, when the samples are stored in liquid nitrogen. Our new approach for measurement of superoxide should greatly simplify the studies of this important radical in biological systems. (C) 2000 Academic Press.

AB - 5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO) is frequently used as a spin trap for the measurement of superoxide by EPR spectrometry. However, its half life is fairly short in room temperature. We here show that superoxide radicals trapped by DEPMPO can be successfully recorded at -196°C. Moreover, we show that the signal intensity remains unaltered for up to 7 days, when the samples are stored in liquid nitrogen. Our new approach for measurement of superoxide should greatly simplify the studies of this important radical in biological systems. (C) 2000 Academic Press.

KW - Allopurinol

KW - DEPMPO

KW - EPR

KW - Liquid nitrogene

KW - Superoxide

KW - Superoxide dismutase

KW - Xanthine oxidase

UR - https://www.scopus.com/pages/publications/0034618730

U2 - 10.1006/bbrc.2000.3387

DO - 10.1006/bbrc.2000.3387

M3 - Article

C2 - 10973818

AN - SCOPUS:0034618730

SN - 0006-291X

VL - 275

SP - 895

EP - 898

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing

Abstract

Keywords*

Field of Science*

Publication Type*

Access to Document

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