Improved method for the preparation of nonyl acridine orange analogues and utilization in detection of cardiolipin

Pavels Dimitrijevs, Ilona Domracheva, Pavel Arsenyan (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

Abstract

This study was designed to develop a fast and convenient methodology for the preparation of 10-nonyl acridine orange (NAO) and its silyl analogues to improve their photo-physical properties for the detection and quantification of cardiolipin (CL). Optimized conditions allow the effective synthesis of NAO analogues with good yield and excellent purity. The introduction of a 3-(trimethylsilyl)propyl moiety improves the dye's solubility and stability in buffer solution and increases its emission intensity by ≈30%. The novel dye can be used for the selective quantification of CL in a liposomal inner mitochondrial membrane model with greater fluorescence intensity and linear slope compared to NAO. The novel silicon-containing NAO analogue has lower cytotoxicity, and is a convenient fluorescent dye for cell staining. This journal is

Original languageEnglish
Pages (from-to)9626-9633
JournalNew Journal of Chemistry
Volume44
Issue number23
DOIs
Publication statusPublished - 21 Jun 2020
Externally publishedYes

Field of Science

  • 1.4 Chemical sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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