Abstract
This study was designed to develop a fast and convenient methodology for the preparation of 10-nonyl acridine orange (NAO) and its silyl analogues to improve their photo-physical properties for the detection and quantification of cardiolipin (CL). Optimized conditions allow the effective synthesis of NAO analogues with good yield and excellent purity. The introduction of a 3-(trimethylsilyl)propyl moiety improves the dye's solubility and stability in buffer solution and increases its emission intensity by ≈30%. The novel dye can be used for the selective quantification of CL in a liposomal inner mitochondrial membrane model with greater fluorescence intensity and linear slope compared to NAO. The novel silicon-containing NAO analogue has lower cytotoxicity, and is a convenient fluorescent dye for cell staining. This journal is
Original language | English |
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Pages (from-to) | 9626-9633 |
Journal | New Journal of Chemistry |
Volume | 44 |
Issue number | 23 |
DOIs | |
Publication status | Published - 21 Jun 2020 |
Externally published | Yes |
Field of Science*
- 1.4 Chemical sciences
Publication Type*
- 1.1. Scientific article indexed in Web of Science and/or Scopus database