In vitro and in vivo examinations for detection of minimal infective dose for biomaterials

A. Reinis, J. Vetra, A. Stunda, L. Berzina-Cimdina, J. Kroica, V. Kuznecova, D. Rostoka

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Citations (Scopus)

Abstract

The objective of the study is to examine the minimal infective dose of Staphylococcus epidermidis and Pseudomonas aeruginosa on biomaterials in vivo and in vitro. In this research originally synthesized bioactive biomaterials - 4N glass and 4NK glass-ceramics are calcium phosphate with Na2O and Nb2O5 additives with different crystallinity. For microbiological investigation of biomaterialsS.epidermidis strain ATCC 12228, Ps.aeruginosa strain ATCC 27853, 4 rabbit animal models, broth and agar, scanned electron microscope Tescan, Vortex centrifuge, sonicator and histological methods were used. We observed that different biomaterials had various attachments of S.epidermidis and Ps.aeruginosa in laboratory animal models. The minimal infective dose of S.epidermidis for biomaterial 4NK is more than 10 3 CFU/ml, for biomaterial 4N less than 103 CFU/ml. The minimal infective dose of Ps.aeruginosa for biomaterial 4N and biomaterial 4NK is less than 103 CFU/ml.

Original languageEnglish
Title of host publication6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Pages1204-1207
Number of pages4
DOIs
Publication statusPublished - 2010
Event6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore, Singapore
Duration: 1 Aug 20106 Aug 2010

Publication series

NameIFMBE Proceedings
Volume31 IFMBE
ISSN (Print)1680-0737

Conference

Conference6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Country/TerritorySingapore
CitySingapore
Period1/08/106/08/10

Keywords*

  • adhesion
  • biomaterials
  • Pseidomonas aeruginosa
  • Staphylococcus aureus

Field of Science*

  • 2.4 Chemical engineering
  • 2.6 Medical engineering

Publication Type*

  • 3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database

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