In vitro and in vivo examinations for detection of minimal infective dose for biomaterials

A. Reinis; J. Vetra; A. Stunda; L. Berzina-Cimdina; J. Kroica; V. Kuznecova; D. Rostoka

doi:10.1007/978-3-642-14515-5_305

In vitro and in vivo examinations for detection of minimal infective dose for biomaterials

A. Reinis, J. Vetra, A. Stunda, L. Berzina-Cimdina, J. Kroica, V. Kuznecova, D. Rostoka

Department of Biology and Microbiology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

The objective of the study is to examine the minimal infective dose of Staphylococcus epidermidis and Pseudomonas aeruginosa on biomaterials in vivo and in vitro. In this research originally synthesized bioactive biomaterials - 4N glass and 4NK glass-ceramics are calcium phosphate with Na₂O and Nb₂O₅ additives with different crystallinity. For microbiological investigation of biomaterialsS.epidermidis strain ATCC 12228, Ps.aeruginosa strain ATCC 27853, 4 rabbit animal models, broth and agar, scanned electron microscope Tescan, Vortex centrifuge, sonicator and histological methods were used. We observed that different biomaterials had various attachments of S.epidermidis and Ps.aeruginosa in laboratory animal models. The minimal infective dose of S.epidermidis for biomaterial 4NK is more than 10 ³ CFU/ml, for biomaterial 4N less than 10³ CFU/ml. The minimal infective dose of Ps.aeruginosa for biomaterial 4N and biomaterial 4NK is less than 10³ CFU/ml.

Original language	English
Title of host publication	6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Pages	1204-1207
Number of pages	4
DOIs	https://doi.org/10.1007/978-3-642-14515-5_305
Publication status	Published - 2010
Event	6th World Congress of Biomechanics (WCB) - Singapore, Singapore Duration: 1 Aug 2010 → 6 Aug 2010 Conference number: 6 https://link.springer.com/book/10.1007/978-3-642-14515-5

Publication series

Name	IFMBE Proceedings
Volume	31 IFMBE
ISSN (Print)	1680-0737

Congress

Congress	6th World Congress of Biomechanics (WCB)
Abbreviated title	WCB 2010
Country/Territory	Singapore
City	Singapore
Period	1/08/10 → 6/08/10
Other	In Conjunction with 14th International Conference on Biomedical Engineering (ICBME) & 5th Asia Pacific Conference on Biomechanics (APBiomech)
Internet address	https://link.springer.com/book/10.1007/978-3-642-14515-5

Keywords*

adhesion
biomaterials
Pseidomonas aeruginosa
Staphylococcus aureus

Field of Science*

2.4 Chemical engineering
2.6 Medical engineering

Publication Type*

3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database

Access to Document

10.1007/978-3-642-14515-5_305

https://link.springer.com/chapter/10.1007/978-3-642-14515-5_305

Cite this

Reinis, A., Vetra, J., Stunda, A., Berzina-Cimdina, L., Kroica, J., Kuznecova, V., & Rostoka, D. (2010). In vitro and in vivo examinations for detection of minimal infective dose for biomaterials. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech (pp. 1204-1207). (IFMBE Proceedings; Vol. 31 IFMBE). https://doi.org/10.1007/978-3-642-14515-5_305

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title = "In vitro and in vivo examinations for detection of minimal infective dose for biomaterials",

abstract = "The objective of the study is to examine the minimal infective dose of Staphylococcus epidermidis and Pseudomonas aeruginosa on biomaterials in vivo and in vitro. In this research originally synthesized bioactive biomaterials - 4N glass and 4NK glass-ceramics are calcium phosphate with Na2O and Nb2O5 additives with different crystallinity. For microbiological investigation of biomaterialsS.epidermidis strain ATCC 12228, Ps.aeruginosa strain ATCC 27853, 4 rabbit animal models, broth and agar, scanned electron microscope Tescan, Vortex centrifuge, sonicator and histological methods were used. We observed that different biomaterials had various attachments of S.epidermidis and Ps.aeruginosa in laboratory animal models. The minimal infective dose of S.epidermidis for biomaterial 4NK is more than 10 3 CFU/ml, for biomaterial 4N less than 103 CFU/ml. The minimal infective dose of Ps.aeruginosa for biomaterial 4N and biomaterial 4NK is less than 103 CFU/ml.",

keywords = "adhesion, biomaterials, Pseidomonas aeruginosa, Staphylococcus aureus",

author = "A. Reinis and J. Vetra and A. Stunda and L. Berzina-Cimdina and J. Kroica and V. Kuznecova and D. Rostoka",

year = "2010",

doi = "10.1007/978-3-642-14515-5_305",

language = "English",

isbn = "9783540790389",

series = "IFMBE Proceedings",

pages = "1204--1207",

booktitle = "6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech",

note = "6th World Congress of Biomechanics (WCB), WCB 2010 ; Conference date: 01-08-2010 Through 06-08-2010",

url = "https://link.springer.com/book/10.1007/978-3-642-14515-5",

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Reinis, A , Vetra, J, Stunda, A, Berzina-Cimdina, L, Kroica, J, Kuznecova, V & Rostoka, D 2010, In vitro and in vivo examinations for detection of minimal infective dose for biomaterials. in 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. IFMBE Proceedings, vol. 31 IFMBE, pp. 1204-1207, 6th World Congress of Biomechanics (WCB), Singapore, Singapore, 1/08/10. https://doi.org/10.1007/978-3-642-14515-5_305

In vitro and in vivo examinations for detection of minimal infective dose for biomaterials. / Reinis, A.; Vetra, J.; Stunda, A. et al.
6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. 2010. p. 1204-1207 (IFMBE Proceedings; Vol. 31 IFMBE).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - In vitro and in vivo examinations for detection of minimal infective dose for biomaterials

AU - Reinis, A.

AU - Vetra, J.

AU - Stunda, A.

AU - Berzina-Cimdina, L.

AU - Kroica, J.

AU - Kuznecova, V.

AU - Rostoka, D.

N1 - Conference code: 6

PY - 2010

Y1 - 2010

N2 - The objective of the study is to examine the minimal infective dose of Staphylococcus epidermidis and Pseudomonas aeruginosa on biomaterials in vivo and in vitro. In this research originally synthesized bioactive biomaterials - 4N glass and 4NK glass-ceramics are calcium phosphate with Na2O and Nb2O5 additives with different crystallinity. For microbiological investigation of biomaterialsS.epidermidis strain ATCC 12228, Ps.aeruginosa strain ATCC 27853, 4 rabbit animal models, broth and agar, scanned electron microscope Tescan, Vortex centrifuge, sonicator and histological methods were used. We observed that different biomaterials had various attachments of S.epidermidis and Ps.aeruginosa in laboratory animal models. The minimal infective dose of S.epidermidis for biomaterial 4NK is more than 10 3 CFU/ml, for biomaterial 4N less than 103 CFU/ml. The minimal infective dose of Ps.aeruginosa for biomaterial 4N and biomaterial 4NK is less than 103 CFU/ml.

AB - The objective of the study is to examine the minimal infective dose of Staphylococcus epidermidis and Pseudomonas aeruginosa on biomaterials in vivo and in vitro. In this research originally synthesized bioactive biomaterials - 4N glass and 4NK glass-ceramics are calcium phosphate with Na2O and Nb2O5 additives with different crystallinity. For microbiological investigation of biomaterialsS.epidermidis strain ATCC 12228, Ps.aeruginosa strain ATCC 27853, 4 rabbit animal models, broth and agar, scanned electron microscope Tescan, Vortex centrifuge, sonicator and histological methods were used. We observed that different biomaterials had various attachments of S.epidermidis and Ps.aeruginosa in laboratory animal models. The minimal infective dose of S.epidermidis for biomaterial 4NK is more than 10 3 CFU/ml, for biomaterial 4N less than 103 CFU/ml. The minimal infective dose of Ps.aeruginosa for biomaterial 4N and biomaterial 4NK is less than 103 CFU/ml.

KW - adhesion

KW - biomaterials

KW - Pseidomonas aeruginosa

KW - Staphylococcus aureus

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U2 - 10.1007/978-3-642-14515-5_305

DO - 10.1007/978-3-642-14515-5_305

M3 - Conference contribution

AN - SCOPUS:77958001248

SN - 9783540790389

T3 - IFMBE Proceedings

SP - 1204

EP - 1207

BT - 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech

T2 - 6th World Congress of Biomechanics (WCB)

Y2 - 1 August 2010 through 6 August 2010

ER -

Reinis A , Vetra J, Stunda A, Berzina-Cimdina L, Kroica J, Kuznecova V et al. In vitro and in vivo examinations for detection of minimal infective dose for biomaterials. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech. 2010. p. 1204-1207. (IFMBE Proceedings). doi: 10.1007/978-3-642-14515-5_305

In vitro and in vivo examinations for detection of minimal infective dose for biomaterials

Abstract

Publication series

Congress

Keywords*

Field of Science*

Publication Type*

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