In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics

D. Loca; A. Dubnika; A. Reinis; N. Romancikova

doi:10.1007/978-3-642-34197-7_49

In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics

D. Loca, A. Dubnika, A. Reinis, N. Romancikova

Department of Biology and Microbiology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

Calcium phosphate ceramic materials in form of hydroxylapatite (HAp) and β-tricalcium phosphate (TCP) are good candidates for bone substitution and regeneration. Majority of methods used for biphasic calcium phosphate (mixture of HAp and TCP) preparation includes synthesis of calcium deficient apatite, not so often mechanical mixing of HAp and TCP is applied. The aim of this work was to evaluate the effect of HAp/TCP ratio as well as the impact of biphasic calcium phosphate (BCP) preparation technique on osteoblast cell behavior and ceramic antimicrobial properties. Results showed that BCP composite samples supported much higher proliferation rate of osteoblast cells as those of pure HAp or TCP. The best result was observed for BCP samples with HAp/TCP ratio 60/40. BCP preparation technique significantly does not affect the cell attachment on the surface of BCP samples, while influence on colonization intensity of bacteria was observed. Results indicated that the use of pure HAp or BCP ceramics as implant materials can cause less inflammatory risks compared to the pure TCP ceramics.

Original language	English
Title of host publication	International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012
Editors	Yuri Dekhtyar, Alexei Katashev, Linda Lancere
Place of Publication	Rīga
Publisher	Springer
Pages	186-189
Number of pages	4
ISBN (Electronic)	978-3-642-34197-7
ISBN (Print)	9783642341960
DOIs	https://doi.org/10.1007/978-3-642-34197-7_49
Publication status	Published - 2013
Event	International Symposium on Biomedical Engineering and Medical Physics - Riga, Latvia Duration: 10 Oct 2012 → 12 Oct 2012 https://link.springer.com/book/10.1007/978-3-642-34197-7 http://www.bini.rtu.lv/isbemp/

Publication series

Name	IFMBE Proceedings
Volume	38 IFMBE
ISSN (Print)	1680-0737

Symposium

Symposium	International Symposium on Biomedical Engineering and Medical Physics
Abbreviated title	ISBEMP 2012
Country/Territory	Latvia
City	Riga
Period	10/10/12 → 12/10/12
Internet address	https://link.springer.com/book/10.1007/978-3-642-34197-7 http://www.bini.rtu.lv/isbemp/

Keywords*

β-tricalcium phosphate
bioceramics
biphasic calcium phosphate ceramics
hydroxyapatite

Field of Science*

2.6 Medical engineering
2.5 Materials engineering

Publication Type*

3.3. Publications in conference proceedings indexed in Web of Science and/or Scopus database

Access to Document

10.1007/978-3-642-34197-7_49

Cite this

Loca, D., Dubnika, A., Reinis, A., & Romancikova, N. (2013). In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics. In Y. Dekhtyar, A. Katashev, & L. Lancere (Eds.), International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012 (pp. 186-189). (IFMBE Proceedings; Vol. 38 IFMBE). Springer. https://doi.org/10.1007/978-3-642-34197-7_49

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title = "In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics",

abstract = "Calcium phosphate ceramic materials in form of hydroxylapatite (HAp) and β-tricalcium phosphate (TCP) are good candidates for bone substitution and regeneration. Majority of methods used for biphasic calcium phosphate (mixture of HAp and TCP) preparation includes synthesis of calcium deficient apatite, not so often mechanical mixing of HAp and TCP is applied. The aim of this work was to evaluate the effect of HAp/TCP ratio as well as the impact of biphasic calcium phosphate (BCP) preparation technique on osteoblast cell behavior and ceramic antimicrobial properties. Results showed that BCP composite samples supported much higher proliferation rate of osteoblast cells as those of pure HAp or TCP. The best result was observed for BCP samples with HAp/TCP ratio 60/40. BCP preparation technique significantly does not affect the cell attachment on the surface of BCP samples, while influence on colonization intensity of bacteria was observed. Results indicated that the use of pure HAp or BCP ceramics as implant materials can cause less inflammatory risks compared to the pure TCP ceramics.",

keywords = "β-tricalcium phosphate, bioceramics, biphasic calcium phosphate ceramics, hydroxyapatite",

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note = "International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012 ; Conference date: 10-10-2012 Through 12-10-2012",

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Loca, D, Dubnika, A, Reinis, A & Romancikova, N 2013, In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics. in Y Dekhtyar, A Katashev & L Lancere (eds), International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012. IFMBE Proceedings, vol. 38 IFMBE, Springer, Rīga, pp. 186-189, International Symposium on Biomedical Engineering and Medical Physics, Riga, Latvia, 10/10/12. https://doi.org/10.1007/978-3-642-34197-7_49

In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics. / Loca, D.; Dubnika, A.; Reinis, A. et al.
International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012. ed. / Yuri Dekhtyar; Alexei Katashev; Linda Lancere. Rīga: Springer, 2013. p. 186-189 (IFMBE Proceedings; Vol. 38 IFMBE).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics

AU - Loca, D.

AU - Dubnika, A.

AU - Reinis, A.

AU - Romancikova, N.

PY - 2013

Y1 - 2013

N2 - Calcium phosphate ceramic materials in form of hydroxylapatite (HAp) and β-tricalcium phosphate (TCP) are good candidates for bone substitution and regeneration. Majority of methods used for biphasic calcium phosphate (mixture of HAp and TCP) preparation includes synthesis of calcium deficient apatite, not so often mechanical mixing of HAp and TCP is applied. The aim of this work was to evaluate the effect of HAp/TCP ratio as well as the impact of biphasic calcium phosphate (BCP) preparation technique on osteoblast cell behavior and ceramic antimicrobial properties. Results showed that BCP composite samples supported much higher proliferation rate of osteoblast cells as those of pure HAp or TCP. The best result was observed for BCP samples with HAp/TCP ratio 60/40. BCP preparation technique significantly does not affect the cell attachment on the surface of BCP samples, while influence on colonization intensity of bacteria was observed. Results indicated that the use of pure HAp or BCP ceramics as implant materials can cause less inflammatory risks compared to the pure TCP ceramics.

AB - Calcium phosphate ceramic materials in form of hydroxylapatite (HAp) and β-tricalcium phosphate (TCP) are good candidates for bone substitution and regeneration. Majority of methods used for biphasic calcium phosphate (mixture of HAp and TCP) preparation includes synthesis of calcium deficient apatite, not so often mechanical mixing of HAp and TCP is applied. The aim of this work was to evaluate the effect of HAp/TCP ratio as well as the impact of biphasic calcium phosphate (BCP) preparation technique on osteoblast cell behavior and ceramic antimicrobial properties. Results showed that BCP composite samples supported much higher proliferation rate of osteoblast cells as those of pure HAp or TCP. The best result was observed for BCP samples with HAp/TCP ratio 60/40. BCP preparation technique significantly does not affect the cell attachment on the surface of BCP samples, while influence on colonization intensity of bacteria was observed. Results indicated that the use of pure HAp or BCP ceramics as implant materials can cause less inflammatory risks compared to the pure TCP ceramics.

KW - β-tricalcium phosphate

KW - bioceramics

KW - biphasic calcium phosphate ceramics

KW - hydroxyapatite

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SN - 9783642341960

T3 - IFMBE Proceedings

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BT - International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012

A2 - Dekhtyar, Yuri

A2 - Katashev, Alexei

A2 - Lancere, Linda

PB - Springer

CY - Rīga

T2 - International Symposium on Biomedical Engineering and Medical Physics

Y2 - 10 October 2012 through 12 October 2012

ER -

Loca D, Dubnika A, Reinis A, Romancikova N. In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics. In Dekhtyar Y, Katashev A, Lancere L, editors, International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012. Rīga: Springer. 2013. p. 186-189. (IFMBE Proceedings). doi: 10.1007/978-3-642-34197-7_49

In vitro evaluation of osteoblast cell behavior and antimicrobial properties of biphasic calcium phosphate ceramics

Abstract

Publication series

Symposium

Keywords*

Field of Science*

Publication Type*

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