Increased level of polyploidy1, a conserved repressor of CYCLINA2 transcription, controls endoreduplication in Arabidopsis

Takeshi Yoshizumi, Yuko Tsumoto, Tomoko Takiguchi, Noriko Nagata, Yoshiharu Y. Yamamoto, Mika Kawashima, Takanari Ichikawa, Miki Nakazawa, Naoki Yamamoto, Minami Matsui

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

Endoreduplication is a type of cell cycle in which DNA replication continues without cell division. We have isolated several dominant mutants from Arabidopsis thaliana activation tagging lines by flow cytometry. One of the mutants, increased level of polyploidy1-1D (Hp1-1D), showed increased polyploidy in both light- and dark-grown hypocotyls. The corresponding gene of ilp1-1D encodes a protein homologous to the C-terminal region of mammalian GC binding factor. We demonstrate that this protein functions as a transcriptional repressor in vivo. The expression of all members of the CYCLINA2 (CYCA2) family was reduced in an ILP1 overexpressing line, and the mouse (Mus musculus) homolog of ILP1 repressed cyclin A2 expression in mouse NIH3T3 cells. T-DNA insertion mutants of ILP1 showed reduced polyploidy and upregulated all CYCA2 expression. Furthermore, loss of CYCA2;1 expression induces an increase in polyploidy in Arabidopsis. We demonstrate that this protein regulates endoreduplication through control of CYCA2 expression in Arabidopsis.

Original languageEnglish
Pages (from-to)2452-2468
Number of pages17
JournalPlant Cell
Volume18
Issue number10
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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