Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue

Birgitte Nielsen; Vanja Cejvanovic; Gitta Wörtwein; Allan R. Hansen; Kasper K. Marstal; Allan Weimann; Peter N. Bjerring; Flemming Dela; Henrik E. Poulsen; Martin B. Jørgensen

doi:10.1016/j.neulet.2018.09.061

Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue

Birgitte Nielsen (Corresponding Author), Vanja Cejvanovic, Gitta Wörtwein, Allan R. Hansen, Kasper K. Marstal, Allan Weimann, Peter N. Bjerring, Flemming Dela, Henrik E. Poulsen, Martin B. Jørgensen

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Major depressive disorder (MDD) affects 350 million people worldwide and is a serious socio-economic burden. The most efficient treatment of MDD is electroconvulsive therapy (ECT), which has been shown to influence the oxidative status believed to be part of the pathophysiology of MDD. We investigated the effects of chronic electroconvulsive stimulation (ECS) on mitochondrial respiration and mitochondrial hydrogen peroxide production, RNA oxidation, and the content of mitochondria in the piriform cortex of the rat. We found reductions of mitochondrial respiration in respiratory states 2 and 3 by 33% and 32%, respectively, and a 23% reduction in electron transfer capacity. RNA oxidation, as measured by 8-oxo-7,8-dihydroguanosine, was increased by 58%, while mitochondrial production of H ₂ O ₂ was unaffected. The increased oxidative stress may thus be ascribed to extra-mitochondrial sources.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Neuroscience Letters
Volume	690
DOIs	https://doi.org/10.1016/j.neulet.2018.09.061
Publication status	Published - 18 Jan 2019
Externally published	Yes

Keywords*

8-oxoGuo
Hydrogen peroxide
Major depression
Mitochondria
Reactive oxygen species (ROS)

Field of Science*

3.2 Clinical medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1016/j.neulet.2018.09.061

https://www.sciencedirect.com/science/article/pii/S0304394018306657?via%3Dihub

Cite this

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title = "Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue",

abstract = " Major depressive disorder (MDD) affects 350 million people worldwide and is a serious socio-economic burden. The most efficient treatment of MDD is electroconvulsive therapy (ECT), which has been shown to influence the oxidative status believed to be part of the pathophysiology of MDD. We investigated the effects of chronic electroconvulsive stimulation (ECS) on mitochondrial respiration and mitochondrial hydrogen peroxide production, RNA oxidation, and the content of mitochondria in the piriform cortex of the rat. We found reductions of mitochondrial respiration in respiratory states 2 and 3 by 33% and 32%, respectively, and a 23% reduction in electron transfer capacity. RNA oxidation, as measured by 8-oxo-7,8-dihydroguanosine, was increased by 58%, while mitochondrial production of H 2 O 2 was unaffected. The increased oxidative stress may thus be ascribed to extra-mitochondrial sources.",

keywords = "8-oxoGuo, Hydrogen peroxide, Major depression, Mitochondria, Reactive oxygen species (ROS)",

author = "Birgitte Nielsen and Vanja Cejvanovic and Gitta W{\"o}rtwein and Hansen, {Allan R.} and Marstal, {Kasper K.} and Allan Weimann and Bjerring, {Peter N.} and Flemming Dela and Poulsen, {Henrik E.} and J{\o}rgensen, {Martin B.}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2019",

month = jan,

day = "18",

doi = "10.1016/j.neulet.2018.09.061",

language = "English",

volume = "690",

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journal = "Neuroscience Letters",

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TY - JOUR

T1 - Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue

AU - Nielsen, Birgitte

AU - Cejvanovic, Vanja

AU - Wörtwein, Gitta

AU - Hansen, Allan R.

AU - Marstal, Kasper K.

AU - Weimann, Allan

AU - Bjerring, Peter N.

AU - Dela, Flemming

AU - Poulsen, Henrik E.

AU - Jørgensen, Martin B.

PY - 2019/1/18

Y1 - 2019/1/18

N2 - Major depressive disorder (MDD) affects 350 million people worldwide and is a serious socio-economic burden. The most efficient treatment of MDD is electroconvulsive therapy (ECT), which has been shown to influence the oxidative status believed to be part of the pathophysiology of MDD. We investigated the effects of chronic electroconvulsive stimulation (ECS) on mitochondrial respiration and mitochondrial hydrogen peroxide production, RNA oxidation, and the content of mitochondria in the piriform cortex of the rat. We found reductions of mitochondrial respiration in respiratory states 2 and 3 by 33% and 32%, respectively, and a 23% reduction in electron transfer capacity. RNA oxidation, as measured by 8-oxo-7,8-dihydroguanosine, was increased by 58%, while mitochondrial production of H 2 O 2 was unaffected. The increased oxidative stress may thus be ascribed to extra-mitochondrial sources.

AB - Major depressive disorder (MDD) affects 350 million people worldwide and is a serious socio-economic burden. The most efficient treatment of MDD is electroconvulsive therapy (ECT), which has been shown to influence the oxidative status believed to be part of the pathophysiology of MDD. We investigated the effects of chronic electroconvulsive stimulation (ECS) on mitochondrial respiration and mitochondrial hydrogen peroxide production, RNA oxidation, and the content of mitochondria in the piriform cortex of the rat. We found reductions of mitochondrial respiration in respiratory states 2 and 3 by 33% and 32%, respectively, and a 23% reduction in electron transfer capacity. RNA oxidation, as measured by 8-oxo-7,8-dihydroguanosine, was increased by 58%, while mitochondrial production of H 2 O 2 was unaffected. The increased oxidative stress may thus be ascribed to extra-mitochondrial sources.

KW - 8-oxoGuo

KW - Hydrogen peroxide

KW - Major depression

KW - Mitochondria

KW - Reactive oxygen species (ROS)

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DO - 10.1016/j.neulet.2018.09.061

M3 - Article

C2 - 30287304

AN - SCOPUS:85054457763

SN - 0304-3940

VL - 690

SP - 1

EP - 5

JO - Neuroscience Letters

JF - Neuroscience Letters

ER -

Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue

Abstract

Keywords*

Field of Science*

Publication Type*

Access to Document

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