Induction of defense responses in pinus sylvestris seedlings by methyl jasmonate and response to heterobasidion annosum and lophodermium seditiosum inoculation

Ilze Šņepste, Baiba Krivmane, Vilnis Šķipars, Astra Zaluma, Dainis E. Ruņģis (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The induction of defense responses in Pinus sylvestris L. seedlings by methyl jasmonate (MeJA) was investigated in three experiments. Two different MeJA application methods were tested, and induction of defense responses was assayed by seedling inoculation with Heterobasidion annosum (Fr.) Bref. and Lophodermium seditiosum Minter, Staley and Millar. In the first experiment, five-year-old P. sylvestris ramets of one clone were directly treated with MeJA, followed by inoculation with H. annosum. In the second experiment, open-pollinated Scots pine seedlings were treated with MeJA by direct spraying and vaporization, and inoculation with H. annosum was done using a slightly modified protocol. In the third experiment, open-pollinated Scots pine seedlings were treated with MeJA by vaporization and inoculated with L. seditiosum. Direct application of MeJA induced seedling mortality, and in some cases, decreased resistance to inoculation with H. annosum. Application of MeJA by vaporization was less stressful for seedlings, and resulted in increased resistance to both H. annosum and L. seditiosum. In addition, an unforeseen Neodiprion sertifer (Geof-froy) and Hylobius abietis L. infestation provided anecdotal evidence of the efficacy of MeJA in inducing resistance to insect pests as well. Further studies are required on the induction of resistance to additional diseases and pests. Induced resistance could be used as a possible protective mecha-nism for Scots pine seedlings prior to planting during reforestation of stands to increase vitality and survival.

Original languageEnglish
Article number628
Number of pages18
JournalForests
Volume12
Issue number5
DOIs
Publication statusPublished - 14 May 2021
Externally publishedYes

Keywords*

  • Fungal pathogens
  • Gene expression
  • Induced resistance
  • Thaumatin-like protein

Field of Science*

  • 4.1 Agriculture, Forestry, and Fisheries
  • 1.6 Biological sciences

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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