Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase

Sarmite Janceva; Anna Andersone; Liga Lauberte; Natalija Zaharova; Galina Telysheva; Jelena Krasilnikova; Inara Nokalne; Julija Janceva

doi:10.4028/www.scientific.net/KEM.903.34

Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase

Sarmite Janceva, Anna Andersone, Liga Lauberte, Natalija Zaharova, Galina Telysheva, Jelena Krasilnikova, Inara Nokalne, Julija Janceva

Department of Human Physiology and Biochemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Citations (Scopus)

Abstract

The effect of water extracts obtained from bark of growing in Latvia and widely spread in Europe grey alder (Alnus incana), black alder (Alnus glutinosa) and aspen tree (Populus tremula), on the amylase activity in human saliva was evaluated. The extracts were obtained using advanced ACE equipment and distilled hot water as a solvent. The total yields of extractives from bark were rather similar and varied from 16 % to 21 % based on dry bark. However, the content of proantocianidine and salicine derivatives used as diagnostic characteristic for evaluation of effect of extracts on activity of amylase – a glucose-polymers cleavage enzyme-differed significantly. The results of in-vitro tests realized using the model of human gastrointestinal pass have shown that the abovementioned extracts in micro dosages are able to increase significantly activity of amylase. However, this effect is dose dependent and when the dose is exceeded the opposite effect – inhibition of amylase activity-is observed. This effect is explained by increasing of proantocianidins component in the volume of the tested extract dose, because pure proantocianidins, isolated from grey alder bark significantly inhibited activity of amylase. The further investigation is necessary in order to estimate effective and safe dosages for application of extracts providing a guaranteed unambiguous effect of activation or inhibition of amylase activity.

Original language	English
Title of host publication	Materials Science and Applied Chemistry IV
Editors	Maris Turks
Publisher	Trans Tech Publications Ltd
Pages	34-39
Number of pages	6
ISBN (Print)	9783035738490
DOIs	https://doi.org/10.4028/www.scientific.net/KEM.903.34
Publication status	Published - 2021
Event	61st International Scientific Conference of Riga Technical University (RTU): Section of Materials Science and Applied Chemistry 2020 - Riga, Latvia Duration: 23 Oct 2020 → 23 Oct 2020 Conference number: 61 https://msac.rtu.lv/wp-content/uploads/sites/18/2020/10/tezes-2020.pdf https://www.rtu.lv/en/university/for-mass-media/news/open/international-education-european-university-initiative

Publication series

Name	Key Engineering Materials
Volume	903 KEM
ISSN (Print)	1013-9826
ISSN (Electronic)	1662-9795

Conference

Conference	61st International Scientific Conference of Riga Technical University (RTU)
Abbreviated title	MSAC 2020
Country/Territory	Latvia
City	Riga
Period	23/10/20 → 23/10/20
Internet address	https://msac.rtu.lv/wp-content/uploads/sites/18/2020/10/tezes-2020.pdf https://www.rtu.lv/en/university/for-mass-media/news/open/international-education-european-university-initiative

Keywords*

Antioxidant activity
Aspen (Populus tremula)
Biological active compounds
Black alder (Alnus glutinosa)
Extracts
Green extraction
Grey alder (Alnus incana)
Proanthocyanidins
Salicins
α-amylase

Field of Science*

2.5 Materials engineering
2.3 Mechanical engineering

Publication Type*

3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database

Access to Document

10.4028/www.scientific.net/KEM.903.34

Cite this

Janceva, S., Andersone, A., Lauberte, L., Zaharova, N., Telysheva, G., Krasilnikova, J., Nokalne, I., & Janceva, J. (2021). Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase. In M. Turks (Ed.), Materials Science and Applied Chemistry IV (pp. 34-39). (Key Engineering Materials; Vol. 903 KEM). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/KEM.903.34

@inproceedings{31df5d3056d34c80b14dee77ac28a776,

title = "Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase",

abstract = "The effect of water extracts obtained from bark of growing in Latvia and widely spread in Europe grey alder (Alnus incana), black alder (Alnus glutinosa) and aspen tree (Populus tremula), on the amylase activity in human saliva was evaluated. The extracts were obtained using advanced ACE equipment and distilled hot water as a solvent. The total yields of extractives from bark were rather similar and varied from 16 % to 21 % based on dry bark. However, the content of proantocianidine and salicine derivatives used as diagnostic characteristic for evaluation of effect of extracts on activity of amylase – a glucose-polymers cleavage enzyme-differed significantly. The results of in-vitro tests realized using the model of human gastrointestinal pass have shown that the abovementioned extracts in micro dosages are able to increase significantly activity of amylase. However, this effect is dose dependent and when the dose is exceeded the opposite effect – inhibition of amylase activity-is observed. This effect is explained by increasing of proantocianidins component in the volume of the tested extract dose, because pure proantocianidins, isolated from grey alder bark significantly inhibited activity of amylase. The further investigation is necessary in order to estimate effective and safe dosages for application of extracts providing a guaranteed unambiguous effect of activation or inhibition of amylase activity.",

keywords = "Antioxidant activity, Aspen (Populus tremula), Biological active compounds, Black alder (Alnus glutinosa), Extracts, Green extraction, Grey alder (Alnus incana), Proanthocyanidins, Salicins, α-amylase",

author = "Sarmite Janceva and Anna Andersone and Liga Lauberte and Natalija Zaharova and Galina Telysheva and Jelena Krasilnikova and Inara Nokalne and Julija Janceva",

note = "Funding Information: This research was funded by a Bio-economic grant “LignoBark” from the Latvian State Institute of Wood Chemistry and ERAF project nr. 1.1.1.1/18/A/182. Publisher Copyright: {\textcopyright} 2021 Trans Tech Publications Ltd, Switzerland.; 61st International Scientific Conference of Riga Technical University (RTU) : Section of Materials Science and Applied Chemistry 2020, MSAC 2020 ; Conference date: 23-10-2020 Through 23-10-2020",

year = "2021",

doi = "10.4028/www.scientific.net/KEM.903.34",

language = "English",

isbn = "9783035738490",

series = "Key Engineering Materials",

publisher = "Trans Tech Publications Ltd",

pages = "34--39",

editor = "Maris Turks",

booktitle = "Materials Science and Applied Chemistry IV",

url = "https://msac.rtu.lv/wp-content/uploads/sites/18/2020/10/tezes-2020.pdf, https://www.rtu.lv/en/university/for-mass-media/news/open/international-education-european-university-initiative",

}

Janceva, S, Andersone, A, Lauberte, L, Zaharova, N, Telysheva, G, Krasilnikova, J , Nokalne, I & Janceva, J 2021, Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase. in M Turks (ed.), Materials Science and Applied Chemistry IV. Key Engineering Materials, vol. 903 KEM, Trans Tech Publications Ltd, pp. 34-39, 61st International Scientific Conference of Riga Technical University (RTU), Riga, Latvia, 23/10/20. https://doi.org/10.4028/www.scientific.net/KEM.903.34

Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase. / Janceva, Sarmite; Andersone, Anna; Lauberte, Liga et al.
Materials Science and Applied Chemistry IV. ed. / Maris Turks. Trans Tech Publications Ltd, 2021. p. 34-39 (Key Engineering Materials; Vol. 903 KEM).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase

AU - Janceva, Sarmite

AU - Andersone, Anna

AU - Lauberte, Liga

AU - Zaharova, Natalija

AU - Telysheva, Galina

AU - Krasilnikova, Jelena

AU - Nokalne, Inara

AU - Janceva, Julija

N1 - Conference code: 61

PY - 2021

Y1 - 2021

N2 - The effect of water extracts obtained from bark of growing in Latvia and widely spread in Europe grey alder (Alnus incana), black alder (Alnus glutinosa) and aspen tree (Populus tremula), on the amylase activity in human saliva was evaluated. The extracts were obtained using advanced ACE equipment and distilled hot water as a solvent. The total yields of extractives from bark were rather similar and varied from 16 % to 21 % based on dry bark. However, the content of proantocianidine and salicine derivatives used as diagnostic characteristic for evaluation of effect of extracts on activity of amylase – a glucose-polymers cleavage enzyme-differed significantly. The results of in-vitro tests realized using the model of human gastrointestinal pass have shown that the abovementioned extracts in micro dosages are able to increase significantly activity of amylase. However, this effect is dose dependent and when the dose is exceeded the opposite effect – inhibition of amylase activity-is observed. This effect is explained by increasing of proantocianidins component in the volume of the tested extract dose, because pure proantocianidins, isolated from grey alder bark significantly inhibited activity of amylase. The further investigation is necessary in order to estimate effective and safe dosages for application of extracts providing a guaranteed unambiguous effect of activation or inhibition of amylase activity.

AB - The effect of water extracts obtained from bark of growing in Latvia and widely spread in Europe grey alder (Alnus incana), black alder (Alnus glutinosa) and aspen tree (Populus tremula), on the amylase activity in human saliva was evaluated. The extracts were obtained using advanced ACE equipment and distilled hot water as a solvent. The total yields of extractives from bark were rather similar and varied from 16 % to 21 % based on dry bark. However, the content of proantocianidine and salicine derivatives used as diagnostic characteristic for evaluation of effect of extracts on activity of amylase – a glucose-polymers cleavage enzyme-differed significantly. The results of in-vitro tests realized using the model of human gastrointestinal pass have shown that the abovementioned extracts in micro dosages are able to increase significantly activity of amylase. However, this effect is dose dependent and when the dose is exceeded the opposite effect – inhibition of amylase activity-is observed. This effect is explained by increasing of proantocianidins component in the volume of the tested extract dose, because pure proantocianidins, isolated from grey alder bark significantly inhibited activity of amylase. The further investigation is necessary in order to estimate effective and safe dosages for application of extracts providing a guaranteed unambiguous effect of activation or inhibition of amylase activity.

KW - Antioxidant activity

KW - Aspen (Populus tremula)

KW - Biological active compounds

KW - Black alder (Alnus glutinosa)

KW - Extracts

KW - Green extraction

KW - Grey alder (Alnus incana)

KW - Proanthocyanidins

KW - Salicins

KW - α-amylase

UR - http://www.scopus.com/inward/record.url?scp=85120715228&partnerID=8YFLogxK

U2 - 10.4028/www.scientific.net/KEM.903.34

DO - 10.4028/www.scientific.net/KEM.903.34

M3 - Conference contribution

AN - SCOPUS:85120715228

SN - 9783035738490

T3 - Key Engineering Materials

SP - 34

EP - 39

BT - Materials Science and Applied Chemistry IV

A2 - Turks, Maris

PB - Trans Tech Publications Ltd

T2 - 61st International Scientific Conference of Riga Technical University (RTU)

Y2 - 23 October 2020 through 23 October 2020

ER -

Janceva S, Andersone A, Lauberte L, Zaharova N, Telysheva G, Krasilnikova J et al. Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase. In Turks M, editor, Materials Science and Applied Chemistry IV. Trans Tech Publications Ltd. 2021. p. 34-39. (Key Engineering Materials). doi: 10.4028/www.scientific.net/KEM.903.34

Influence of extracts from bark of deciduous trees on the activity of the amylolytic enzyme-alpha amylase

Abstract

Publication series

Conference

Keywords*

Field of Science*

Publication Type*

Access to Document

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