Interferon gamma production in whole peripheral blood culture in acute hepatitis B

N. Osna, A. Duk, A. Sochnev

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Interferon gamma (IFNg) production in whole peripheral blood (WPB) and mononuclear (MN) cell culture in acute hepatitis B (AHB) was compared. IFNg production was induced by phytogem agglutinin and measured in the cell supernatants of 14 AHB patients in the course of the disease. There were some up-regulating factors of IFNg production that probably operated in WPB culture: the presence of autoerythrocytes as well as the low content of monocytes. Autoserum regulated IFNg production in a stage-dependent way: it decreased IFNg activity at the bilirubin peak in hepatitis B infection, but not in convalescence. In contrast, we did not find a serum blocking effect in the corresponding stage of acute hepatitis A. The nature of this serum blocking factor in AHB is unclear.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalScandinavian Journal of Clinical and Laboratory Investigation
Volume56
Issue number2
DOIs
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • cell culture
  • HBV-infection
  • interferon gamma induction
  • serum blocking factor

Field of Science

  • 1.4 Chemical sciences
  • 1.6 Biological sciences
  • 3.2 Clinical medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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