Investigation of mrsa isolates from pig orgin, environemnt and stuffby SPA typing, antimirobial resistance and se gene detection

MRSA (methicillin resistant Staphylococcus aureus) is hard curable and highly resistant zoonotic soft tissue pathogen which is also capable to produce enterotoxins and cause food toxiconfections. The aim of the study was the characterization of MRSA isolates from different pig farms and slaughterhouses based on interaction of antimicrobial resistance, spa type, SE genes and source of MRSA isolates. In total 405 pigs, 105 carcasses, 34 workers as well as 46 samples from environment from several farms and slaughterhouses were examined by conventional microbial and molecular methods. In total 155 (14.6%) MRSA isolates were detected from 1064 samples tested and included 11 positive pigs (27.4%) form all tested pigs, 2 isolates (4.3%) from environment, 3 isolates (4.3%) from milk, 7isolates (6.7%) from pig carcasses and 7 isolates (20.6%) from workers. From all MRSA isolates 48.4% contained sea, 1.3% seb, 5.9% sec and 9.7% seh. Study showed that sea mostly appears alone (70.4%) in MRSA isolates or together with seh (14.8%), but seh trends to be together in isolate genome with other genes that are coding SE. In the same time in these MRSA isolates sec appears alone or together with sea. Contrary to other investigations, our study indicated high sea distribution in MRSA isolates and only some isolates with sec, moreover among MRSA spa types with high sea distribution antimicrobial resistance was lower.