Laser speckle imaging for visualization of hidden effects for early detection of antibacterial susceptibility in disc diffusion tests

Ilya Balmages; Aigars Reinis; Svjatoslavs Kistkins; Dmitrijs Bliznuks; Emilija V. Plorina; Alexey Lihachev; Ilze Lihacova

doi:10.3389/fmicb.2023.1221134

Laser speckle imaging for visualization of hidden effects for early detection of antibacterial susceptibility in disc diffusion tests

Ilya Balmages (Corresponding Author), Aigars Reinis, Svjatoslavs Kistkins, Dmitrijs Bliznuks, Emilija V. Plorina, Alexey Lihachev, Ilze Lihacova

Department of Biology and Microbiology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

5 Downloads (Pure)

Abstract

Rapid identification of effective antibiotic treatment is crucial for increasing patient survival and preventing the formation of new antibiotic-resistant bacteria due to preventative antibiotic use. Currently utilized “gold standard” methods require 16–24 h to determine the most appropriate antibiotic for the patient’s treatment. The proposed technique of laser speckle imaging with subpixel correlation analysis allows for identifying dynamics and changes in the zone of inhibition, which are impossible to observe with classical methods. Furthermore, it obtains the resulting zone of inhibition diameter earlier than the disk diffusion method which is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). These results could improve mathematical models of changes in the diameter of the zone of inhibition around the disc containing the antimicrobial agent, thereby speeding up and facilitating epidemiological analysis.

Original language	English
Article number	1221134
Number of pages	10
Journal	Frontiers in Microbiology
Volume	14
DOIs	https://doi.org/10.3389/fmicb.2023.1221134
Publication status	Published - 29 Jun 2023

Field of Science*

1.6 Biological sciences
3.4 Medical biotechnology
3.1 Basic medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.3389/fmicb.2023.1221134Licence: CC BY

Laser speckle imaging for visualization of hiddenFinal published version, 2.86 MBLicence: CC BY

Cite this

@article{ddc0c94ba55942b1a949a6f1f49f4a73,

title = "Laser speckle imaging for visualization of hidden effects for early detection of antibacterial susceptibility in disc diffusion tests",

abstract = "Rapid identification of effective antibiotic treatment is crucial for increasing patient survival and preventing the formation of new antibiotic-resistant bacteria due to preventative antibiotic use. Currently utilized “gold standard” methods require 16–24 h to determine the most appropriate antibiotic for the patient{\textquoteright}s treatment. The proposed technique of laser speckle imaging with subpixel correlation analysis allows for identifying dynamics and changes in the zone of inhibition, which are impossible to observe with classical methods. Furthermore, it obtains the resulting zone of inhibition diameter earlier than the disk diffusion method which is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). These results could improve mathematical models of changes in the diameter of the zone of inhibition around the disc containing the antimicrobial agent, thereby speeding up and facilitating epidemiological analysis.",

author = "Ilya Balmages and Aigars Reinis and Svjatoslavs Kistkins and Dmitrijs Bliznuks and Plorina, {Emilija V.} and Alexey Lihachev and Ilze Lihacova",

note = "Funding Information: This work has been supported by the European Regional Development Fund project “Rapid assessment system of antibacterial resistance for patients with secondary bacterial infections” (No. 1.1.1.1/21/A/034). Publisher Copyright: Copyright {\textcopyright} 2023 Balmages, Reinis, Kistkins, Bliznuks, Plorina, Lihachev and Lihacova.",

year = "2023",

month = jun,

day = "29",

doi = "10.3389/fmicb.2023.1221134",

language = "English",

volume = "14",

journal = "Frontiers in Microbiology",

issn = "1664-302X",

publisher = "Frontiers Media S.A.",

}

TY - JOUR

T1 - Laser speckle imaging for visualization of hidden effects for early detection of antibacterial susceptibility in disc diffusion tests

AU - Balmages, Ilya

AU - Reinis, Aigars

AU - Kistkins, Svjatoslavs

AU - Bliznuks, Dmitrijs

AU - Plorina, Emilija V.

AU - Lihachev, Alexey

AU - Lihacova, Ilze

N1 - Funding Information: This work has been supported by the European Regional Development Fund project “Rapid assessment system of antibacterial resistance for patients with secondary bacterial infections” (No. 1.1.1.1/21/A/034). Publisher Copyright: Copyright © 2023 Balmages, Reinis, Kistkins, Bliznuks, Plorina, Lihachev and Lihacova.

PY - 2023/6/29

Y1 - 2023/6/29

N2 - Rapid identification of effective antibiotic treatment is crucial for increasing patient survival and preventing the formation of new antibiotic-resistant bacteria due to preventative antibiotic use. Currently utilized “gold standard” methods require 16–24 h to determine the most appropriate antibiotic for the patient’s treatment. The proposed technique of laser speckle imaging with subpixel correlation analysis allows for identifying dynamics and changes in the zone of inhibition, which are impossible to observe with classical methods. Furthermore, it obtains the resulting zone of inhibition diameter earlier than the disk diffusion method which is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). These results could improve mathematical models of changes in the diameter of the zone of inhibition around the disc containing the antimicrobial agent, thereby speeding up and facilitating epidemiological analysis.

AB - Rapid identification of effective antibiotic treatment is crucial for increasing patient survival and preventing the formation of new antibiotic-resistant bacteria due to preventative antibiotic use. Currently utilized “gold standard” methods require 16–24 h to determine the most appropriate antibiotic for the patient’s treatment. The proposed technique of laser speckle imaging with subpixel correlation analysis allows for identifying dynamics and changes in the zone of inhibition, which are impossible to observe with classical methods. Furthermore, it obtains the resulting zone of inhibition diameter earlier than the disk diffusion method which is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). These results could improve mathematical models of changes in the diameter of the zone of inhibition around the disc containing the antimicrobial agent, thereby speeding up and facilitating epidemiological analysis.

UR - http://www.scopus.com/inward/record.url?scp=85164936458&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2023.1221134

DO - 10.3389/fmicb.2023.1221134

M3 - Article

SN - 1664-302X

VL - 14

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 1221134

ER -

Laser speckle imaging for visualization of hidden effects for early detection of antibacterial susceptibility in disc diffusion tests

Abstract

Field of Science*

Publication Type*

Access to Document

Other files and links

Fingerprint

Corrigendum: Laser speckle imaging for visualization of hidden effects for early detection of antibacterial susceptibility in disc diffusion tests (Frontiers in Microbiology, (2023), 14, (1221134), 10.3389/fmicb.2023.1221134)

Cite this