Local and systemic morphofunctional response of osteoporotic rabbits bone defect following implantation of strontium doped biphasic ceramic granules

Jānis Zariņš, Mara Pilmane, Elga Sidhoma, Ilze Salma, Janis Locs

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)


Variety of different bone substitutive materials are synthetized to improve bone healing potentials in pathological bone conditions. Physiologically active molecules within biomaterials, can initiate expression level of biomarkers, regulating bone remodeling. Aim of our study was to analyze bone healing process in bone defects followed by implantation with 5 % strontium substituted hydroxyapatite (HAP)/tricalcium phosphate (TCP) 70/30 granules (group A) or HAP/TCP biphasic ceramic granules without strontium substitution (group B), or sham surgery affected bone (group C) in osteoporotic rabbits’ femur. Tissue samples from contralateral intact left leg were used for evaluation of systemic effects after surgery. Changes of bone volume were measured and appearance of OPG, NFkB-105, OC, COL-1, BMP-2/4, MMP-2, TIMP-2, IL-1 and IL-10-positive osteocytes in osteoporotic rabbits’ bone defect were evaluated. No statistical difference between groups of trabecular bone volume was detected. All analyzed markers showed higher appearance of positive osteocytes in groups A and B with comparison to control left leg (p < 0.05). Only NFkB105-positive cells showed important difference between sham surgery affected leg and control one (p = 0.034). Numerous OPG-positive cells appeared in group A, while moderate number of them was found in groups B and C (p = 0.025; p = 0.027). Numerous to abundant OC-positive osteocytes were detected in group A, while moderate in group C (p = 0.034). Statistical difference of rest biomarkers between groups was not detected. We concluded that implantation of biomaterials in osteoporotic bone improves local bone regenerative properties. However, the notable increase of OPG-containing cells proves the increase of osteoclastogenesis suppression and gives the evidence for renew of bone functionality.

Original languageEnglish
Title of host publicationMaterials Engineering 2017 - 26th International Baltic Conference on Materials Engineering
EditorsRegita Bendikiene, Kazimieras Juzenas
PublisherTrans Tech Publications Ltd
Number of pages8
ISBN (Print)9783035711479
Publication statusPublished - 2017
Event26th International Baltic Conference "Materials Engineering 2017" - Kaunas, Lithuania
Duration: 26 Oct 201727 Oct 2017
Conference number: 26

Publication series

NameSolid State Phenomena
Volume267 SSP
ISSN (Print)1012-0394
ISSN (Electronic)1662-9779


Conference26th International Baltic Conference "Materials Engineering 2017"
Internet address


  • Biomaterials
  • Bone regeneration
  • Factors
  • Osteoporosis
  • Rabbit
  • Strontium

Field of Science*

  • 1.3 Physical sciences
  • 2.5 Materials engineering

Publication Type*

  • 3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database


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