Lunasin-induced behavioural effects in mice: Focus on the dopaminergic system

Zane Dzirkale, Juris Rumaks, Simons Svirskis, Olga Mazina, Anni Allikalt, Ago Rinken, Kaspars Jekabsons, Ruta Muceniece, Vija Klusa

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The present study for the first time is devoted to identify central effects of synthetic lunasin, a 43 amino acid peptide. A markedly expressed neuroleptic/cataleptic effect was observed at low (0.1–10 nmol/mouse) centrally administered doses in male C57Bl/6 mice. Lunasin considerably reduced the amphetamine hyperlocomotion but weakly apomorphine climbing behaviour. No influence on ketamine and bicuculline effects was observed. Binding assay studies demonstrated modest affinity of lunasin for the dopamine D1 receptor (Ki = 60 ± 15 μM). In a functional assay of cAMP accumulation on live cells lunasin antagonised apomorphine effect on D1 receptor activation (pEC50 = 6.1 ± 0.3), but had no effect in cells expressing D2 receptors. The obtained data suggest that lunasin's action at least in part is provided via dopaminergic D1 receptor pathways. However, other non-identified mechanisms (probably intracellular) may play an important role in lunasin's central action. Nevertheless further studies of lunasin are promising, particularly taking into account a necessity for novel type of antipsychotic drugs.
Original languageEnglish
Pages (from-to)5-9
Number of pages5
JournalBehavioural Brain Research
Volume256
DOIs
Publication statusPublished - 1 Nov 2013
Externally publishedYes

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 1.3. Anonymously reviewed scientific article published in a journal with an international editorial board and is available in another indexed database

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