TY - JOUR
T1 - Magnetic levitational bioassembly of 3D tissue construct in space
AU - Parfenov, Vladislav A.
AU - Khesuani, Yusef D.
AU - Petrov, Stanislav V.
AU - Karalkin, Pavel A.
AU - Koudan, Elizaveta V.
AU - Nezhurina, Elizaveta K.
AU - Pereira, Frederico D.A.S.
AU - Krokhmal, Alisa A.
AU - Gryadunova, Anna A.
AU - Bulanova, Elena A.
AU - Vakhrushev, Igor V.
AU - Babichenko, Igor I.
AU - Kasjanovs, Vladimirs
AU - Petrov, Oleg F.
AU - Vasiliev, Mikhail M.
AU - Brakke, Kenn
AU - Belousov, Sergei I.
AU - Grigoriev, Timofei E.
AU - Osidak, Egor O.
AU - Rossiyskaya, Ekaterina I.
AU - Buravkova, Ludmila B.
AU - Kononenko, Oleg D.
AU - Demirci, Utkan
AU - Mironov, Vladimir A.
N1 - Funding Information:
The study was supported by the RFBR (research project no. 18-29-11076).
Publisher Copyright:
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/7
Y1 - 2020/7
N2 - Magnetic levitational bioassembly of three-dimensional (3D) tissue constructs represents a rapidly emerging scaffold- and label-free approach and alternative conceptual advance in tissue engineering. The magnetic bioassembler has been designed, developed, and certified for life space research. To the best of our knowledge, 3D tissue constructs have been biofabricated for the first time in space under microgravity from tissue spheroids consisting of human chondrocytes. Bioassembly and sequential tissue spheroid fusion presented a good agreement with developed predictive mathematical models and computer simulations. Tissue constructs demonstrated good viability and advanced stages of tissue spheroid fusion process. Thus, our data strongly suggest that scaffold-free formative biofabrication using magnetic fields is a feasible alternative to traditional scaffold-based approaches, hinting a new perspective avenue of research that could significantly advance tissue engineering. Magnetic levitational bioassembly in space can also advance space life science and space regenerative medicine.
AB - Magnetic levitational bioassembly of three-dimensional (3D) tissue constructs represents a rapidly emerging scaffold- and label-free approach and alternative conceptual advance in tissue engineering. The magnetic bioassembler has been designed, developed, and certified for life space research. To the best of our knowledge, 3D tissue constructs have been biofabricated for the first time in space under microgravity from tissue spheroids consisting of human chondrocytes. Bioassembly and sequential tissue spheroid fusion presented a good agreement with developed predictive mathematical models and computer simulations. Tissue constructs demonstrated good viability and advanced stages of tissue spheroid fusion process. Thus, our data strongly suggest that scaffold-free formative biofabrication using magnetic fields is a feasible alternative to traditional scaffold-based approaches, hinting a new perspective avenue of research that could significantly advance tissue engineering. Magnetic levitational bioassembly in space can also advance space life science and space regenerative medicine.
UR - http://www.scopus.com/inward/record.url?scp=85088908733&partnerID=8YFLogxK
U2 - 10.1126/sciadv.aba4174
DO - 10.1126/sciadv.aba4174
M3 - Article
C2 - 32743068
AN - SCOPUS:85088908733
SN - 2375-2548
VL - 6
JO - Science advances
JF - Science advances
IS - 29
M1 - eaba4174
ER -