Magnetic Patterning of Tissue Spheroids Using Polymer Microcapsules Containing Iron Oxide Nanoparticles

Elizaveta V Koudan (Coresponding Author), Mikhail N Zharkov, Mikhail V Gerasimov, Saida Sh Karshieva, Aleksandra D Shirshova, Vladimir V Chrishtop, Vladimir Kasyanov, Aleksandr A Levin, Vladislav A Parfenov, Pavel A Karalkin, Frederico D A S Pereira, Stanislav V Petrov, Nikolay A Pyataev, Yusef D Khesuani, Vladimir A Mironov, Gleb B Sukhorukov

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Magnetic tissue engineering is one of the rapidly emerging and promising directions of tissue engineering and biofabrication where the magnetic field is employed as temporal removal support or scaffold. Iron oxide nanoparticles are used to label living cells and provide the desired magnetic properties. Recently, polymer microcapsules loaded with iron oxide nanoparticles have been proposed as a novel approach to designing magnetic materials with high local concentrations. These microcapsules can be readily internalized and retained intracellularly for a long time in various types of cells. The low cytotoxicity of these microcapsules was previously shown in 2D cell culture. This paper has demonstrated that cells containing these nontoxic nanomaterials can form viable 3D tissue spheroids for the first time. The spheroids retained labeled fluorescent microcapsules with magnetic nanoparticles without a detectable cytotoxic effect. The high concentration of packed nanoparticles inside the microcapsules enables the evident magnetic properties of the labeled spheroids to be maintained. Finally, magnetic spheroids can be effectively used for magnetic patterning and biofabrication of tissue-engineering constructs.

Original languageEnglish
Pages (from-to)5206 -5214
Number of pages9
JournalACS Biomaterials Science and Engineering
Volume7
Issue number11
DOIs
Publication statusPublished - 8 Nov 2021

Field of Science*

  • 2.9 Industrial biotechnology
  • 3.1 Basic medicine
  • 2.5 Materials engineering

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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