Major histocompatibility complex class I chain related (MIC) a gene, TNFa microsatellite alleles and TNFB alleles in juvenile idiopathic arthritis patients from Latvia

Liene Nikitina Zake, Ija Cimdina, Ingrida Rumba, Preethi Dabadghao, Carani B. Sanjeevi

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

In order to analyze involvement of major histocompatibility complex class I chain-related gene A (MICA) and tumor necrosis factor a (TNFa) microsatellite polymorphisms as well as TNFB gene in juvenile idiopathic arthritis (JIA), we studied 128 patients divided into groups according to clinical features [monoarthritis (n = 14), oligoarthritis (n = 58), polyarthritis (n = 50), and systemic (n = 6)], and 114 age- and sex-matched healthy controls from Latvia. DNA samples were amplified with specific primers and used for genotyping of MICA and TNFa microsatellite. Typing for a biallelic NcoI polymerase chain reaction RFLP polymorphism located at the first intron of TNFB gene was done as follows: restriction digests generated fragments of 555bp and 185bp for TNFB*1 allele, and 740bp for TNFB*2 allele. The results were compared between cases and controls. We found significant increase of MICA allele A4 (p = 0.009; odds ratio [OR] = 2,3) and allele TNFa2 (p = 0.0001; OR = 4.4) in patients compared with controls. The frequency of allele TNFa9 was significantly decreased (p = 0.0001; OR = 0.1) in patients with JIA. No significant differences of TNFB allele frequency were found. Our data suggest that MICA and TNFa microsatellite polymorphisms may be used as markers for determination of susceptibility and protection from JIA.

Original languageEnglish
Pages (from-to)418-423
Number of pages6
JournalHuman Immunology
Volume63
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • JIA
  • MICA
  • TNFa
  • TNFB

Field of Science

  • 3.1 Basic medicine
  • 3.2 Clinical medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

Fingerprint

Dive into the research topics of 'Major histocompatibility complex class I chain related (MIC) a gene, TNFa microsatellite alleles and TNFB alleles in juvenile idiopathic arthritis patients from Latvia'. Together they form a unique fingerprint.

Cite this