Metagenomics in ophthalmology: Current findings and future prospectives

Davide Borroni; Vito Romano; Stephen B. Kaye; Tobi Somerville; Luca Napoli; Adriano Fasolo; Paola Gallon; Diego Ponzin; Alfonso Esposito; Stefano Ferrari

doi:10.1136/bmjophth-2018-000248

Metagenomics in ophthalmology: Current findings and future prospectives

Davide Borroni, Vito Romano, Stephen B. Kaye, Tobi Somerville, Luca Napoli, Adriano Fasolo, Paola Gallon, Diego Ponzin, Alfonso Esposito, Stefano Ferrari

Research output: Contribution to journal › Review article › peer-review

50 Citations (Scopus)

Abstract

Less than 1% of all microorganisms of the available environmental microbiota can be cultured with the currently available techniques. Metagenomics is a new methodology of high-throughput DNA sequencing, able to provide taxonomic and functional profiles of microbial communities without the necessity to culture microbes in the laboratory. Metagenomics opens to a a € hypothesis-free' approach, giving important details for future research and treatment of ocular diseases in ophthalmology, such as ocular infection and ocular surface diseases.

Original language	English
Article number	e000248
Journal	BMJ Open Ophthalmology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1136/bmjophth-2018-000248
Publication status	Published - 1 Jun 2019

Keywords*

cornea
eye
metagenomics
microbiome
ocular surface
ophthalmology
sequencing

Field of Science*

3.2 Clinical medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1136/bmjophth-2018-000248

Cite this

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abstract = "Less than 1% of all microorganisms of the available environmental microbiota can be cultured with the currently available techniques. Metagenomics is a new methodology of high-throughput DNA sequencing, able to provide taxonomic and functional profiles of microbial communities without the necessity to culture microbes in the laboratory. Metagenomics opens to a a € hypothesis-free' approach, giving important details for future research and treatment of ocular diseases in ophthalmology, such as ocular infection and ocular surface diseases.",

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T2 - Current findings and future prospectives

AU - Borroni, Davide

AU - Romano, Vito

AU - Kaye, Stephen B.

AU - Somerville, Tobi

AU - Napoli, Luca

AU - Fasolo, Adriano

AU - Gallon, Paola

AU - Ponzin, Diego

AU - Esposito, Alfonso

AU - Ferrari, Stefano

N1 - Publisher Copyright: © Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Less than 1% of all microorganisms of the available environmental microbiota can be cultured with the currently available techniques. Metagenomics is a new methodology of high-throughput DNA sequencing, able to provide taxonomic and functional profiles of microbial communities without the necessity to culture microbes in the laboratory. Metagenomics opens to a a € hypothesis-free' approach, giving important details for future research and treatment of ocular diseases in ophthalmology, such as ocular infection and ocular surface diseases.

AB - Less than 1% of all microorganisms of the available environmental microbiota can be cultured with the currently available techniques. Metagenomics is a new methodology of high-throughput DNA sequencing, able to provide taxonomic and functional profiles of microbial communities without the necessity to culture microbes in the laboratory. Metagenomics opens to a a € hypothesis-free' approach, giving important details for future research and treatment of ocular diseases in ophthalmology, such as ocular infection and ocular surface diseases.

KW - cornea

KW - eye

KW - metagenomics

KW - microbiome

KW - ocular surface

KW - ophthalmology

KW - sequencing

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DO - 10.1136/bmjophth-2018-000248

M3 - Review article

AN - SCOPUS:85067057012

SN - 2397-3269

VL - 4

JO - BMJ Open Ophthalmology

JF - BMJ Open Ophthalmology

IS - 1

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ER -

Metagenomics in ophthalmology: Current findings and future prospectives

Abstract

Keywords*

Field of Science*

Publication Type*

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