Metagenomics in ophthalmology: Current findings and future prospectives

Davide Borroni; Vito Romano; Stephen B. Kaye; Tobi Somerville; Luca Napoli; Adriano Fasolo; Paola Gallon; Diego Ponzin; Alfonso Esposito; Stefano Ferrari

doi:10.1136/bmjophth-2018-000248

Metagenomics in ophthalmology: Current findings and future prospectives

Davide Borroni
, Vito Romano
, Stephen B. Kaye
, Tobi Somerville
, Luca Napoli
, Adriano Fasolo
, Paola Gallon
, Diego Ponzin
, Alfonso Esposito
, Stefano Ferrari

Research output: Contribution to journal › Review article › peer-review

64 Citations (Scopus)

Abstract

Less than 1% of all microorganisms of the available environmental microbiota can be cultured with the currently available techniques. Metagenomics is a new methodology of high-throughput DNA sequencing, able to provide taxonomic and functional profiles of microbial communities without the necessity to culture microbes in the laboratory. Metagenomics opens to a a € hypothesis-free' approach, giving important details for future research and treatment of ocular diseases in ophthalmology, such as ocular infection and ocular surface diseases.

Original language	English
Article number	e000248
Journal	BMJ Open Ophthalmology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1136/bmjophth-2018-000248
Publication status	Published - 1 Jun 2019

Keywords*

cornea
eye
metagenomics
microbiome
ocular surface
ophthalmology
sequencing

Field of Science*

3.2 Clinical medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1136/bmjophth-2018-000248

Cite this

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title = "Metagenomics in ophthalmology: Current findings and future prospectives",

abstract = "Less than 1\% of all microorganisms of the available environmental microbiota can be cultured with the currently available techniques. Metagenomics is a new methodology of high-throughput DNA sequencing, able to provide taxonomic and functional profiles of microbial communities without the necessity to culture microbes in the laboratory. Metagenomics opens to a a € hypothesis-free' approach, giving important details for future research and treatment of ocular diseases in ophthalmology, such as ocular infection and ocular surface diseases.",

keywords = "cornea, eye, metagenomics, microbiome, ocular surface, ophthalmology, sequencing",

author = "Davide Borroni and Vito Romano and Kaye, \{Stephen B.\} and Tobi Somerville and Luca Napoli and Adriano Fasolo and Paola Gallon and Diego Ponzin and Alfonso Esposito and Stefano Ferrari",

note = "Publisher Copyright: {\textcopyright} Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.",

year = "2019",

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doi = "10.1136/bmjophth-2018-000248",

language = "English",

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journal = "BMJ Open Ophthalmology",

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TY - JOUR

T1 - Metagenomics in ophthalmology

T2 - Current findings and future prospectives

AU - Borroni, Davide

AU - Romano, Vito

AU - Kaye, Stephen B.

AU - Somerville, Tobi

AU - Napoli, Luca

AU - Fasolo, Adriano

AU - Gallon, Paola

AU - Ponzin, Diego

AU - Esposito, Alfonso

AU - Ferrari, Stefano

N1 - Publisher Copyright: © Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Less than 1% of all microorganisms of the available environmental microbiota can be cultured with the currently available techniques. Metagenomics is a new methodology of high-throughput DNA sequencing, able to provide taxonomic and functional profiles of microbial communities without the necessity to culture microbes in the laboratory. Metagenomics opens to a a € hypothesis-free' approach, giving important details for future research and treatment of ocular diseases in ophthalmology, such as ocular infection and ocular surface diseases.

AB - Less than 1% of all microorganisms of the available environmental microbiota can be cultured with the currently available techniques. Metagenomics is a new methodology of high-throughput DNA sequencing, able to provide taxonomic and functional profiles of microbial communities without the necessity to culture microbes in the laboratory. Metagenomics opens to a a € hypothesis-free' approach, giving important details for future research and treatment of ocular diseases in ophthalmology, such as ocular infection and ocular surface diseases.

KW - cornea

KW - eye

KW - metagenomics

KW - microbiome

KW - ocular surface

KW - ophthalmology

KW - sequencing

UR - https://www.scopus.com/pages/publications/85067057012

U2 - 10.1136/bmjophth-2018-000248

DO - 10.1136/bmjophth-2018-000248

M3 - Review article

AN - SCOPUS:85067057012

SN - 2397-3269

VL - 4

JO - BMJ Open Ophthalmology

JF - BMJ Open Ophthalmology

IS - 1

M1 - e000248

ER -

Metagenomics in ophthalmology: Current findings and future prospectives

Abstract

Keywords*

Field of Science*

Publication Type*

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