Metagenomics in ophthalmology: Hypothesis or real prospective?

Paola Gallon; Mohit Parekh; Stefano Ferrari; Adriano Fasolo; Diego Ponzin; Davide Borroni

doi:10.1016/j.btre.2019.e00355

Metagenomics in ophthalmology: Hypothesis or real prospective?

Paola Gallon, Mohit Parekh, Stefano Ferrari, Adriano Fasolo, Diego Ponzin, Davide Borroni

Research output: Contribution to journal › Review article › peer-review

34 Citations (Scopus)

Abstract

Metagenomic analysis was originally associated with the studies of genetic material from environmental samples. But, with the advent of the Human Microbiome Project, it has now been applied in clinical practices. The ocular surface (OS) is the most exposed part of the eye, colonized by several microbial communities (both, OS and environmental) that contribute to the maintenance of the physiological state. Limited knowledge has been acquired on these microbes due to the limitations of conventional diagnostic methods. Emerging fields of research are focusing on Next Generation Sequencing (NGS) technologies to obtain reliable information on the OS microbiome. Currently only pre-specified pathogens can be detected by conventional culture-based techniques or Polymerase Chain Reaction (PCR), but there are conditions to state whether metagenomics could revolutionize the diagnosis of ocular diseases. The aim of this review is to provide an updated overview of the studies involving NGS technology for OS microbiome.

Original language	English
Article number	e00355
Journal	Biotechnology Reports
Volume	23
DOIs	https://doi.org/10.1016/j.btre.2019.e00355
Publication status	Published - Sept 2019

Keywords*

Metagenomics
Microbiome
NGS (next generation sequencing)
Ocular infection
Ocular surface

Field of Science*

3.2 Clinical medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.btre.2019.e00355

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title = "Metagenomics in ophthalmology: Hypothesis or real prospective?",

abstract = "Metagenomic analysis was originally associated with the studies of genetic material from environmental samples. But, with the advent of the Human Microbiome Project, it has now been applied in clinical practices. The ocular surface (OS) is the most exposed part of the eye, colonized by several microbial communities (both, OS and environmental) that contribute to the maintenance of the physiological state. Limited knowledge has been acquired on these microbes due to the limitations of conventional diagnostic methods. Emerging fields of research are focusing on Next Generation Sequencing (NGS) technologies to obtain reliable information on the OS microbiome. Currently only pre-specified pathogens can be detected by conventional culture-based techniques or Polymerase Chain Reaction (PCR), but there are conditions to state whether metagenomics could revolutionize the diagnosis of ocular diseases. The aim of this review is to provide an updated overview of the studies involving NGS technology for OS microbiome.",

keywords = "Metagenomics, Microbiome, NGS (next generation sequencing), Ocular infection, Ocular surface",

author = "Paola Gallon and Mohit Parekh and Stefano Ferrari and Adriano Fasolo and Diego Ponzin and Davide Borroni",

note = "Funding Information: The authors thank Mrs. Jaini Parekh for the illustration of Figs. 1 and 2 . The authors thank the European Society of Cataract & Refractive Surgeons for the META-COR grant (PI: Davide Borroni), funded through the ESCRS clinical research trial funding programme 2017-2018. Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

month = sep,

doi = "10.1016/j.btre.2019.e00355",

language = "English",

volume = "23",

journal = "Biotechnology Reports",

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T2 - Hypothesis or real prospective?

AU - Gallon, Paola

AU - Parekh, Mohit

AU - Ferrari, Stefano

AU - Fasolo, Adriano

AU - Ponzin, Diego

AU - Borroni, Davide

N1 - Funding Information: The authors thank Mrs. Jaini Parekh for the illustration of Figs. 1 and 2 . The authors thank the European Society of Cataract & Refractive Surgeons for the META-COR grant (PI: Davide Borroni), funded through the ESCRS clinical research trial funding programme 2017-2018. Publisher Copyright: © 2019 The Authors

PY - 2019/9

Y1 - 2019/9

N2 - Metagenomic analysis was originally associated with the studies of genetic material from environmental samples. But, with the advent of the Human Microbiome Project, it has now been applied in clinical practices. The ocular surface (OS) is the most exposed part of the eye, colonized by several microbial communities (both, OS and environmental) that contribute to the maintenance of the physiological state. Limited knowledge has been acquired on these microbes due to the limitations of conventional diagnostic methods. Emerging fields of research are focusing on Next Generation Sequencing (NGS) technologies to obtain reliable information on the OS microbiome. Currently only pre-specified pathogens can be detected by conventional culture-based techniques or Polymerase Chain Reaction (PCR), but there are conditions to state whether metagenomics could revolutionize the diagnosis of ocular diseases. The aim of this review is to provide an updated overview of the studies involving NGS technology for OS microbiome.

AB - Metagenomic analysis was originally associated with the studies of genetic material from environmental samples. But, with the advent of the Human Microbiome Project, it has now been applied in clinical practices. The ocular surface (OS) is the most exposed part of the eye, colonized by several microbial communities (both, OS and environmental) that contribute to the maintenance of the physiological state. Limited knowledge has been acquired on these microbes due to the limitations of conventional diagnostic methods. Emerging fields of research are focusing on Next Generation Sequencing (NGS) technologies to obtain reliable information on the OS microbiome. Currently only pre-specified pathogens can be detected by conventional culture-based techniques or Polymerase Chain Reaction (PCR), but there are conditions to state whether metagenomics could revolutionize the diagnosis of ocular diseases. The aim of this review is to provide an updated overview of the studies involving NGS technology for OS microbiome.

KW - Metagenomics

KW - Microbiome

KW - NGS (next generation sequencing)

KW - Ocular infection

KW - Ocular surface

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DO - 10.1016/j.btre.2019.e00355

M3 - Review article

AN - SCOPUS:85068170110

SN - 2215-017X

VL - 23

JO - Biotechnology Reports

JF - Biotechnology Reports

M1 - e00355

ER -

Metagenomics in ophthalmology: Hypothesis or real prospective?

Abstract

Keywords*

Field of Science*

Publication Type*

UN SDGs

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