Method for obtaining plasmid DNA by miniprep method

Mihails Bariševs; Dmitrijs Merkulovs; Ivans Mironovs; Vitalijs  Mironovs

Method for obtaining plasmid DNA by miniprep method

Translated title of the contribution: Plazmīdas DNS iegūšanas paņēmiens pēc miniprep metodes

Mihails Bariševs (Inventor), Dmitrijs Merkulovs (Inventor), Ivans Mironovs (Inventor), Vitalijs Mironovs (Inventor)

Rīga Stradiņš University

Research output: Intellectual property › Patent

Abstract

The invention relates to biotechnology, in particular to the production of pure plasmid
DNA, which can be used in the analysis of DNA methylation. Obtaining pure plasmid DNA includes precipitationof a bacterial culture Escherichia coli, resuspension of the sediment, preparation of an alkaline lysate, neutralization of an alkaline lysate, separation of cell debris, binding of plasmid DNA, washing of plasmid DNA with a buffer, and elution of plasmid DNA.

Translated title of the contribution	Plazmīdas DNS iegūšanas paņēmiens pēc miniprep metodes
Original language	Latvian
Patent number	15565
IPC	C12N 15/11,C12N 1/21
Priority date	2/02/21
Filing date	2/02/21
Publication status	Published - 20 Nov 2021

Keywords*

plasmid DNA

Field of Science*

2.8 Environmental biotechnology

Patent expiration date

02.02.2041

Access to Document

http://databases.lrpv.gov.lv/databases/lv/patent/details/lvp2021000006/

Cite this

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title = "Method for obtaining plasmid DNA by miniprep method",

abstract = "The invention relates to biotechnology, in particular to the production of pure plasmid DNA, which can be used in the analysis of DNA methylation. Obtaining pure plasmid DNA includes precipitationof a bacterial culture Escherichia coli, resuspension of the sediment, preparation of an alkaline lysate, neutralization of an alkaline lysate, separation of cell debris, binding of plasmid DNA, washing of plasmid DNA with a buffer, and elution of plasmid DNA.",

keywords = "plasmid DNA",

author = "Mihails Bari{\v s}evs and Dmitrijs Merkulovs and Ivans Mironovs and Vitalijs Mironovs",

year = "2021",

month = nov,

day = "20",

language = "Latvian",

publisher = "Patent Office of the Republic of Latvia",

type = "Patent",

note = "15565; C12N 15/11,C12N 1/21",

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TY - PAT

T1 - Method for obtaining plasmid DNA by miniprep method

AU - Bariševs, Mihails

AU - Merkulovs, Dmitrijs

AU - Mironovs, Ivans

AU - Mironovs, Vitalijs

PY - 2021/11/20

Y1 - 2021/11/20

N2 - The invention relates to biotechnology, in particular to the production of pure plasmid DNA, which can be used in the analysis of DNA methylation. Obtaining pure plasmid DNA includes precipitationof a bacterial culture Escherichia coli, resuspension of the sediment, preparation of an alkaline lysate, neutralization of an alkaline lysate, separation of cell debris, binding of plasmid DNA, washing of plasmid DNA with a buffer, and elution of plasmid DNA.

AB - The invention relates to biotechnology, in particular to the production of pure plasmid DNA, which can be used in the analysis of DNA methylation. Obtaining pure plasmid DNA includes precipitationof a bacterial culture Escherichia coli, resuspension of the sediment, preparation of an alkaline lysate, neutralization of an alkaline lysate, separation of cell debris, binding of plasmid DNA, washing of plasmid DNA with a buffer, and elution of plasmid DNA.

KW - plasmid DNA

UR - https://www.lrpv.gov.lv/lv/media/2102/downloa

M3 - Patent

M1 - 15565

Y2 - 2021/02/02

PB - Patent Office of the Republic of Latvia

ER -

Method for obtaining plasmid DNA by miniprep method

Abstract

Keywords*

Field of Science*

Patent expiration date

Access to Document

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