Method for obtaining plasmid DNA by miniprep method

Translated title of the contribution: Plazmīdas DNS iegūšanas paņēmiens pēc miniprep metodes

Mihails Bariševs (Inventor), Dmitrijs Merkulovs (Inventor), Ivans Mironovs (Inventor), Vitalijs Mironovs (Inventor)

Research output: Intellectual propertyPatent


The invention relates to biotechnology, in particular to the production of pure plasmid
DNA, which can be used in the analysis of DNA methylation. Obtaining pure plasmid DNA includes precipitationof a bacterial culture Escherichia coli, resuspension of the sediment, preparation of an alkaline lysate, neutralization of an alkaline lysate, separation of cell debris, binding of plasmid DNA, washing of plasmid DNA with a buffer, and elution of plasmid DNA.
Translated title of the contributionPlazmīdas DNS iegūšanas paņēmiens pēc miniprep metodes
Original languageLatvian
Patent number15565
IPCC12N 15/11,C12N 1/21
Priority date2/02/21
Filing date2/02/21
Publication statusPublished - 20 Nov 2021


  • plasmid DNA

Field of Science*

  • 2.8 Environmental biotechnology

Patent expiration date

  • 02.02.2041


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