Abstract
Objective: Mismatch repair (MMR) deficiency is present in ≈ 15 % of
colorectal carcinoma (CRC) cases, including Lynch syndrome(LS)-related cancers (Whitehall et al.,2011; Berginc et al.,2009). In turn, a relationship between epithelial-mesenchymal transition (EMT) and LS has been
demonstrated (Gu et al.,2014). Our aim was to investigate EMT-MMR
protein association in consecutive CRCs.
Method: By immunohistochemistry, expression of MSH2, MSH6,
PMS2, MLH1, CD44, E-cadherin was detected. Expression intensity EI
was measured in a 0–3 scale (0, no expression; 1-weak, 2-moderate, 3-
strong). The final score was expressed as a sum of EIs by extent (%).
Results: Evaluating retrospectively 124 consecutive CRCs, loss of MSH2,
MSH6, PMS2, and MLH1 was found in 1;0;11 and 4 cases, respectively.
The MMR protein expression was reclassified as low versus high using the
median as cut-off: MSH2 1.39; MSH6 1.9; PMS2 1.8; MLH1 1.43. The
overall expression of E-cadherin was 1.81[95 % confidence interval: 1.74–
1.89], of CD44: 1.29[1.16–1.41]. T test showed statistically significant
difference in E-cadherin level by MSH2 (low 1.72[1.61–1.82]; high
1.91[1.81–2.01]; p = 0.01) or PMS2 (low 1.71[1.60–1.82]; high
1.95[1.85–2.05]; p < 0.01). CD44 showed significant difference by
PMS2 (low 1.43[1.24–1.62]; high 1.17[1.00–1.34]; p = 0.04).
Conclusion: In the study, significant, complex associations are shown
between EMT and expression level of MMR proteins in consecutive CRC.
colorectal carcinoma (CRC) cases, including Lynch syndrome(LS)-related cancers (Whitehall et al.,2011; Berginc et al.,2009). In turn, a relationship between epithelial-mesenchymal transition (EMT) and LS has been
demonstrated (Gu et al.,2014). Our aim was to investigate EMT-MMR
protein association in consecutive CRCs.
Method: By immunohistochemistry, expression of MSH2, MSH6,
PMS2, MLH1, CD44, E-cadherin was detected. Expression intensity EI
was measured in a 0–3 scale (0, no expression; 1-weak, 2-moderate, 3-
strong). The final score was expressed as a sum of EIs by extent (%).
Results: Evaluating retrospectively 124 consecutive CRCs, loss of MSH2,
MSH6, PMS2, and MLH1 was found in 1;0;11 and 4 cases, respectively.
The MMR protein expression was reclassified as low versus high using the
median as cut-off: MSH2 1.39; MSH6 1.9; PMS2 1.8; MLH1 1.43. The
overall expression of E-cadherin was 1.81[95 % confidence interval: 1.74–
1.89], of CD44: 1.29[1.16–1.41]. T test showed statistically significant
difference in E-cadherin level by MSH2 (low 1.72[1.61–1.82]; high
1.91[1.81–2.01]; p = 0.01) or PMS2 (low 1.71[1.60–1.82]; high
1.95[1.85–2.05]; p < 0.01). CD44 showed significant difference by
PMS2 (low 1.43[1.24–1.62]; high 1.17[1.00–1.34]; p = 0.04).
Conclusion: In the study, significant, complex associations are shown
between EMT and expression level of MMR proteins in consecutive CRC.
| Original language | English |
|---|---|
| Article number | PS-13-015 |
| Pages (from-to) | S179-S179 |
| Number of pages | 1 |
| Journal | Virchows Archiv |
| Volume | 471 |
| Issue number | Suppl.1 |
| Publication status | Published - Sept 2017 |
| Event | 29th European Congress of Pathology: Pathology for patient care - Amsterdam, Netherlands Duration: 2 Sept 2017 → 6 Sept 2017 Conference number: 29 https://pathology.nl/evenement/29th-european-congress-of-pathology/ https://www.esp-congress.org/_Resources/Persistent/c6589e10208b86a3700a8c1f36aca4325f15f3a4/ECP2017_Final%20Programme.pdf |
Field of Science*
- 3.1 Basic medicine
- 3.2 Clinical medicine
Publication Type*
- 3.3. Publications in conference proceedings indexed in Web of Science and/or Scopus database
