Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism

Agris Pentjušs, Egils Stalidzāns (Coresponding Author), Jānis Liepiņš, Agnese Kokina, Jekaterina Martinova, Pēteris Zikmanis, Ivars Mozga, Rita Ščerbaka, H. Hartman, M. G. Poolman, D. A. Fell, Armands Vīgants

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism’s potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.

Original languageEnglish
Pages (from-to)1177-1190
Number of pages14
JournalJournal of Industrial Microbiology and Biotechnology
Issue number8
Publication statusPublished - 1 Aug 2017
Externally publishedYes


  • Central metabolism
  • Essentiality analysis
  • Kluyveromyces marxianus
  • Metabolic engineering
  • Modelling

Field of Science*

  • 3.4 Medical biotechnology

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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