Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism

Agris Pentjušs; Egils Stalidzāns; Jānis Liepiņš; Agnese Kokina; Jekaterina Martinova; Pēteris Zikmanis; Ivars Mozga; Rita Ščerbaka; H. Hartman; M. G. Poolman; D. A. Fell; Armands Vīgants

doi:10.1007/s10295-017-1946-8

Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism

Agris Pentjušs, Egils Stalidzāns (Corresponding Author), Jānis Liepiņš, Agnese Kokina, Jekaterina Martinova, Pēteris Zikmanis, Ivars Mozga, Rita Ščerbaka, H. Hartman, M. G. Poolman, D. A. Fell, Armands Vīgants

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Abstract

The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism’s potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.

Original language	English
Pages (from-to)	1177-1190
Number of pages	14
Journal	Journal of Industrial Microbiology and Biotechnology
Volume	44
Issue number	8
DOIs	https://doi.org/10.1007/s10295-017-1946-8
Publication status	Published - 1 Aug 2017
Externally published	Yes

Keywords*

Central metabolism
Essentiality analysis
Kluyveromyces marxianus
Metabolic engineering
Modelling

Field of Science*

3.4 Medical biotechnology

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

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10.1007/s10295-017-1946-8

Model-based biotechnological potential analysisFinal published version, 1.32 MB

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Pentjušs, A., Stalidzāns, E., Liepiņš, J., Kokina, A., Martinova, J., Zikmanis, P., Mozga, I., Ščerbaka, R., Hartman, H., Poolman, M. G., Fell, D. A., & Vīgants, A. (2017). Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism. Journal of Industrial Microbiology and Biotechnology, 44(8), 1177-1190. https://doi.org/10.1007/s10295-017-1946-8

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title = "Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism",

abstract = "The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism{\textquoteright}s potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.",

keywords = "Central metabolism, Essentiality analysis, Kluyveromyces marxianus, Metabolic engineering, Modelling",

author = "Agris Pentju{\v s}s and Egils Stalidzāns and Jānis Liepiņ{\v s} and Agnese Kokina and Jekaterina Martinova and Pēteris Zikmanis and Ivars Mozga and Rita {\v S}{\v c}erbaka and H. Hartman and Poolman, {M. G.} and Fell, {D. A.} and Armands Vīgants",

note = "Funding Information: The research was supported by ERDF project Nr. 2DP/2.1.1.1.0/14/APIA/VIAA/043. Publisher Copyright: {\textcopyright} 2017, Society for Industrial Microbiology and Biotechnology.",

year = "2017",

month = aug,

day = "1",

doi = "10.1007/s10295-017-1946-8",

language = "English",

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journal = "Journal of Industrial Microbiology and Biotechnology",

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Pentjušs, A, Stalidzāns, E, Liepiņš, J, Kokina, A, Martinova, J, Zikmanis, P, Mozga, I, Ščerbaka, R, Hartman, H, Poolman, MG, Fell, DA & Vīgants, A 2017, 'Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism', Journal of Industrial Microbiology and Biotechnology, vol. 44, no. 8, pp. 1177-1190. https://doi.org/10.1007/s10295-017-1946-8

TY - JOUR

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AU - Pentjušs, Agris

AU - Stalidzāns, Egils

AU - Liepiņš, Jānis

AU - Kokina, Agnese

AU - Martinova, Jekaterina

AU - Zikmanis, Pēteris

AU - Mozga, Ivars

AU - Ščerbaka, Rita

AU - Hartman, H.

AU - Poolman, M. G.

AU - Fell, D. A.

AU - Vīgants, Armands

PY - 2017/8/1

Y1 - 2017/8/1

N2 - The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism’s potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.

AB - The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism’s potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.

KW - Central metabolism

KW - Essentiality analysis

KW - Kluyveromyces marxianus

KW - Metabolic engineering

KW - Modelling

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DO - 10.1007/s10295-017-1946-8

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C2 - 28444480

AN - SCOPUS:85018670164

SN - 1367-5435

VL - 44

SP - 1177

EP - 1190

JO - Journal of Industrial Microbiology and Biotechnology

JF - Journal of Industrial Microbiology and Biotechnology

IS - 8

ER -

Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism

Abstract

Keywords*

Field of Science*

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