Monocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditis

Liba Sokolovska; Alina Sultanova; Maksims Čistjakovs; Egils Cunskis; Modra Murovska

doi:10.2478/prolas-2019-0018

Monocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditis

Liba Sokolovska, Alina Sultanova, Maksims Čistjakovs, Egils Cunskis, Modra Murovska

Institute of Microbiology and Virology

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Abstract

The aim of this study was to investigate the possibility of using monocytes/macrophages as mediators in human herpesvirus-6 (HHV-6) infection of thyroid gland tissues in autoimmune thyroiditis (AIT). Seventy-three AIT patients were enrolled in this study. The control group consisted of 80 blood donors. Monocyte/macrophage isolation for AIT patient samples was performed by adherence. HHV-6 was detected in peripheral blood mononuclear cell (PBMC) DNA samples using nested polymerase chain reaction (nPCR). Gene expression of HHV-6 active infection marker (U79/80) and chemokine receptors (U12, U51) in patient monocyte/macrophage samples and blood donor PBMC samples was detected using reverse-transcription PCR. HHV-6 viral load was detected by using quantitative-PCR technique. The HHV-6 genomic sequence was found significantly more frequently among AIT patient than control group samples. Markers of active infection were found in 8 AIT patient monocyte/macrophage samples (11%) and in none of control group PBMC samples. HHV-6 U51 mRNA expression was detected only in AIT patient samples (2/24 previously positive for HHV-6). Since HHV-6 genomic sequences were found significantly more frequently in AIT patient samples and active infection markers were found in patient monocytes/macrophages, our results suggest that monocytes/macrophages may be used by HHV-6 as mediators for thyroid gland infection.

Original language	English
Pages (from-to)	112-116
Number of pages	5
Journal	Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
Volume	73
Issue number	2
DOIs	https://doi.org/10.2478/prolas-2019-0018
Publication status	Published - 1 May 2019

Keywords*

autoimmunity
HHV-6
thyroid gland

Field of Science*

1.6 Biological sciences
3.2 Clinical medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.2478/prolas-2019-0018

Monocytes-Macrophages Act as Mediators for Human HerpesvirusLicence: CC BY-NC-ND

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title = "Monocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditis",

abstract = "The aim of this study was to investigate the possibility of using monocytes/macrophages as mediators in human herpesvirus-6 (HHV-6) infection of thyroid gland tissues in autoimmune thyroiditis (AIT). Seventy-three AIT patients were enrolled in this study. The control group consisted of 80 blood donors. Monocyte/macrophage isolation for AIT patient samples was performed by adherence. HHV-6 was detected in peripheral blood mononuclear cell (PBMC) DNA samples using nested polymerase chain reaction (nPCR). Gene expression of HHV-6 active infection marker (U79/80) and chemokine receptors (U12, U51) in patient monocyte/macrophage samples and blood donor PBMC samples was detected using reverse-transcription PCR. HHV-6 viral load was detected by using quantitative-PCR technique. The HHV-6 genomic sequence was found significantly more frequently among AIT patient than control group samples. Markers of active infection were found in 8 AIT patient monocyte/macrophage samples (11%) and in none of control group PBMC samples. HHV-6 U51 mRNA expression was detected only in AIT patient samples (2/24 previously positive for HHV-6). Since HHV-6 genomic sequences were found significantly more frequently in AIT patient samples and active infection markers were found in patient monocytes/macrophages, our results suggest that monocytes/macrophages may be used by HHV-6 as mediators for thyroid gland infection.",

keywords = "autoimmunity, HHV-6, thyroid gland",

author = "Liba Sokolovska and Alina Sultanova and Maksims {\v C}istjakovs and Egils Cunskis and Modra Murovska",

note = "Funding Information: This work was supported by the National Research Programme “Biomedicine for the Public Health” (BIOMEDICINE) Publisher Copyright: {\textcopyright} 2019 Liba Sokolovska et al., published by Sciendo 2019. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

year = "2019",

month = may,

day = "1",

doi = "10.2478/prolas-2019-0018",

language = "English",

volume = "73",

pages = "112--116",

journal = "Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences",

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Sokolovska, L , Sultanova, A , Čistjakovs, M, Cunskis, E & Murovska, M 2019, 'Monocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditis', Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, vol. 73, no. 2, pp. 112-116. https://doi.org/10.2478/prolas-2019-0018

Monocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditis. / Sokolovska, Liba ; Sultanova, Alina ; Čistjakovs, Maksims et al.
In: Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, Vol. 73, No. 2, 01.05.2019, p. 112-116.

Research output: Contribution to journal › Article › peer-review

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AU - Sokolovska, Liba

AU - Sultanova, Alina

AU - Čistjakovs, Maksims

AU - Cunskis, Egils

AU - Murovska, Modra

N1 - Funding Information: This work was supported by the National Research Programme “Biomedicine for the Public Health” (BIOMEDICINE) Publisher Copyright: © 2019 Liba Sokolovska et al., published by Sciendo 2019. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - The aim of this study was to investigate the possibility of using monocytes/macrophages as mediators in human herpesvirus-6 (HHV-6) infection of thyroid gland tissues in autoimmune thyroiditis (AIT). Seventy-three AIT patients were enrolled in this study. The control group consisted of 80 blood donors. Monocyte/macrophage isolation for AIT patient samples was performed by adherence. HHV-6 was detected in peripheral blood mononuclear cell (PBMC) DNA samples using nested polymerase chain reaction (nPCR). Gene expression of HHV-6 active infection marker (U79/80) and chemokine receptors (U12, U51) in patient monocyte/macrophage samples and blood donor PBMC samples was detected using reverse-transcription PCR. HHV-6 viral load was detected by using quantitative-PCR technique. The HHV-6 genomic sequence was found significantly more frequently among AIT patient than control group samples. Markers of active infection were found in 8 AIT patient monocyte/macrophage samples (11%) and in none of control group PBMC samples. HHV-6 U51 mRNA expression was detected only in AIT patient samples (2/24 previously positive for HHV-6). Since HHV-6 genomic sequences were found significantly more frequently in AIT patient samples and active infection markers were found in patient monocytes/macrophages, our results suggest that monocytes/macrophages may be used by HHV-6 as mediators for thyroid gland infection.

AB - The aim of this study was to investigate the possibility of using monocytes/macrophages as mediators in human herpesvirus-6 (HHV-6) infection of thyroid gland tissues in autoimmune thyroiditis (AIT). Seventy-three AIT patients were enrolled in this study. The control group consisted of 80 blood donors. Monocyte/macrophage isolation for AIT patient samples was performed by adherence. HHV-6 was detected in peripheral blood mononuclear cell (PBMC) DNA samples using nested polymerase chain reaction (nPCR). Gene expression of HHV-6 active infection marker (U79/80) and chemokine receptors (U12, U51) in patient monocyte/macrophage samples and blood donor PBMC samples was detected using reverse-transcription PCR. HHV-6 viral load was detected by using quantitative-PCR technique. The HHV-6 genomic sequence was found significantly more frequently among AIT patient than control group samples. Markers of active infection were found in 8 AIT patient monocyte/macrophage samples (11%) and in none of control group PBMC samples. HHV-6 U51 mRNA expression was detected only in AIT patient samples (2/24 previously positive for HHV-6). Since HHV-6 genomic sequences were found significantly more frequently in AIT patient samples and active infection markers were found in patient monocytes/macrophages, our results suggest that monocytes/macrophages may be used by HHV-6 as mediators for thyroid gland infection.

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ER -

Monocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditis

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