Nonrandom loss of human chromosome 3 fragments from mouse‐human microcell hybrids following progressive growth in SCID mice

Stephan Imreh, Irina Kholodnyuk, Rando Allikmetts, Eric J. Stanbridge, Eugene R. Zabarovsky, George Klein

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Microcell hybrid lines of A9 mouse fibrosarcoma containing complete or partially deleted human chromosomes 3 (chr. 3) were inoculated into SCID mice. Cell lines derived from the tumors were examined by fluorescent in situ hybridization for the status of the transferred human chromosome and by PCR for marker loss. The SCID tumors arising after the inoculation of 105 cells were passaged serially in vivo and regularly showed loss of four markers; D3S1029 (3p21.3–21.2), AP20R (3p22–21.3.) D3S32 (3p21.3‐p21.2), and THRB (3p24). This regularly deleted region is bordered by markers GNA12 (3p21.1‐p21.3) and VHL (3p25) that were maintained in a fraction of tumors. Fragments derived from the long arm of chromosome 3 and corresponding markers in the 3q26‐q28 region were retained in all tumors. Our findings may be related to the postulated presence of tumor suppressor genes in the 3p24‐p21 region as indicated by the frequent deletion of this region in renal and small cell lung carcinomas and other solid tumors. The technically cumbersome identification of suppressor genes may be supplemented by an “elimination test” based on analogous principles.

Original languageEnglish
Pages (from-to)237-245
Number of pages9
JournalGenes, Chromosomes and Cancer
Volume11
Issue number4
DOIs
Publication statusPublished - Dec 1994
Externally publishedYes

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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