Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study

Carlos Rocha-de-Lossada; Cosimo Mazzotta; Federico Gabrielli; Filomena Tiziana Papa; Carmen Gómez-Huertas; Celia García-López; Facundo Urbinati; Rahul Rachwani-Anil; María García-Lorente; José María Sánchez-González; Miguel Rechichi; Giovanni Rubegni; Davide Borroni

doi:10.3390/jcm12196354

Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study

Carlos Rocha-de-Lossada, Cosimo Mazzotta, Federico Gabrielli, Filomena Tiziana Papa, Carmen Gómez-Huertas, Celia García-López, Facundo Urbinati, Rahul Rachwani-Anil, María García-Lorente, José María Sánchez-González, Miguel Rechichi, Giovanni Rubegni, Davide Borroni (Corresponding Author)

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of naïve keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development.

Original language	English
Article number	6354
Journal	Journal of clinical medicine
Volume	12
Issue number	19
DOIs	https://doi.org/10.3390/jcm12196354
Publication status	Published - Oct 2023
Externally published	Yes

Keywords*

keratoconus
metagenomics
microbiota
validation-study

Field of Science*

3.2 Clinical medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

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10.3390/jcm12196354Licence: CC BY

Ocular Surface Microbiota in Naïve KeratoconusFinal published version, 2.14 MBLicence: CC BY

Cite this

Rocha-de-Lossada, C., Mazzotta, C., Gabrielli, F., Papa, F. T., Gómez-Huertas, C., García-López, C., Urbinati, F., Rachwani-Anil, R., García-Lorente, M., Sánchez-González, J. M., Rechichi, M., Rubegni, G., & Borroni, D. (2023). Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study. Journal of clinical medicine, 12(19), Article 6354. https://doi.org/10.3390/jcm12196354

@article{fe033cf389d3430a874bc776a79c08c1,

title = "Ocular Surface Microbiota in Na{\"i}ve Keratoconus: A Multicenter Validation Study",

abstract = "In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of na{\"i}ve keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development.",

keywords = "keratoconus, metagenomics, microbiota, validation-study",

author = "Carlos Rocha-de-Lossada and Cosimo Mazzotta and Federico Gabrielli and Papa, {Filomena Tiziana} and Carmen G{\'o}mez-Huertas and Celia Garc{\'i}a-L{\'o}pez and Facundo Urbinati and Rahul Rachwani-Anil and Mar{\'i}a Garc{\'i}a-Lorente and S{\'a}nchez-Gonz{\'a}lez, {Jos{\'e} Mar{\'i}a} and Miguel Rechichi and Giovanni Rubegni and Davide Borroni",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = oct,

doi = "10.3390/jcm12196354",

language = "English",

volume = "12",

journal = "Journal of clinical medicine",

issn = "2077-0383",

publisher = "MDPI AG",

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Rocha-de-Lossada, C, Mazzotta, C, Gabrielli, F, Papa, FT, Gómez-Huertas, C, García-López, C, Urbinati, F, Rachwani-Anil, R, García-Lorente, M, Sánchez-González, JM, Rechichi, M, Rubegni, G & Borroni, D 2023, 'Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study', Journal of clinical medicine, vol. 12, no. 19, 6354. https://doi.org/10.3390/jcm12196354

TY - JOUR

T1 - Ocular Surface Microbiota in Naïve Keratoconus

T2 - A Multicenter Validation Study

AU - Rocha-de-Lossada, Carlos

AU - Mazzotta, Cosimo

AU - Gabrielli, Federico

AU - Papa, Filomena Tiziana

AU - Gómez-Huertas, Carmen

AU - García-López, Celia

AU - Urbinati, Facundo

AU - Rachwani-Anil, Rahul

AU - García-Lorente, María

AU - Sánchez-González, José María

AU - Rechichi, Miguel

AU - Rubegni, Giovanni

AU - Borroni, Davide

PY - 2023/10

Y1 - 2023/10

N2 - In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of naïve keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development.

AB - In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of naïve keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development.

KW - keratoconus

KW - metagenomics

KW - microbiota

KW - validation-study

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U2 - 10.3390/jcm12196354

DO - 10.3390/jcm12196354

M3 - Article

AN - SCOPUS:85173828520

SN - 2077-0383

VL - 12

JO - Journal of clinical medicine

JF - Journal of clinical medicine

IS - 19

M1 - 6354

ER -

Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study

Abstract

Keywords*

Field of Science*

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