TY - JOUR
T1 - Oregonin from Alnus incana bark affects DNA methyltransferases expression and mitochondrial DNA copies in mouse embryonic fibroblasts
AU - Krasilnikova, Jelena
AU - Lauberte, Liga
AU - Stoyanova, Elena
AU - Abadjieva, Desislava
AU - Chervenkov, Mihail
AU - Mori, Mattia
AU - De Paolis, Elisa
AU - Mladenova, Vanya
AU - Telysheva, Galina
AU - Botta, Bruno
AU - Kistanova, Elena
N1 - Funding Information:
This work was supported by National Scientific Fund of Bulgaria [Grant DKOST01/10]. The research was implemented under frame of collaboration in COST Action FA1403 and bilateral project between Bulgarian Academy of Sciences and Latvian Academy of Sciences. We gratitude doctor M. Kicheva from “Progene-Ltd” company for technical support of the gene expression and mtDNA copy research.
Funding Information:
This work was supported by National Scientific Fund of Bulgaria [Grant DKOST01/10].
Publisher Copyright:
© 2018, © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Oregonin is an open-chain diarylheptanoid isolated from Alnus incana bark that possesses remarkable antioxidant and anti-inflammatory properties, inhibits adipogenesis, and can be used in the prevention of obesity and related metabolic disorders. Here, we aimed to investigate the effects of oregonin on the epigenetic regulation in cells as well as its ability to modulate DNA methylating enzymes expression and mitochondrial DNA (mtDNA) copies. Our results show that oregonin altered the expression of DNA methyltransferases and mtDNA copy numbers in dependency on concentration and specificity of cells genotype. A close correlation between mtDNA copy numbers and mRNA expression of the mtDnmt1 and Dnmt3b was established. Moreover, molecular modeling suggested that oregonin fits the catalytic site of DNMT1 and partially overlaps with binding of the cofactor. These findings further extend the knowledge on oregonin, and elucidate for the first time its potential to affect the key players of the DNA methylation process, namely DNMTs transcripts and mtDNA.
AB - Oregonin is an open-chain diarylheptanoid isolated from Alnus incana bark that possesses remarkable antioxidant and anti-inflammatory properties, inhibits adipogenesis, and can be used in the prevention of obesity and related metabolic disorders. Here, we aimed to investigate the effects of oregonin on the epigenetic regulation in cells as well as its ability to modulate DNA methylating enzymes expression and mitochondrial DNA (mtDNA) copies. Our results show that oregonin altered the expression of DNA methyltransferases and mtDNA copy numbers in dependency on concentration and specificity of cells genotype. A close correlation between mtDNA copy numbers and mRNA expression of the mtDnmt1 and Dnmt3b was established. Moreover, molecular modeling suggested that oregonin fits the catalytic site of DNMT1 and partially overlaps with binding of the cofactor. These findings further extend the knowledge on oregonin, and elucidate for the first time its potential to affect the key players of the DNA methylation process, namely DNMTs transcripts and mtDNA.
KW - Alnus incana bark
KW - DNA methyltransferases mRNAs
KW - mtDNA copy
KW - Oregonin
UR - http://www.scopus.com/inward/record.url?scp=85048256882&partnerID=8YFLogxK
U2 - 10.1080/14756366.2018.1476504
DO - 10.1080/14756366.2018.1476504
M3 - Article
C2 - 29877148
AN - SCOPUS:85048256882
SN - 1475-6366
VL - 33
SP - 1055
EP - 1063
JO - Journal of Enzyme Inhibition and Medicinal Chemistry
JF - Journal of Enzyme Inhibition and Medicinal Chemistry
IS - 1
ER -