Organ printing: Computer-aided jet printer-based three-dimensional tissue engineering

Vladimir Mironov; Thomas Boland; Chris Wilson; Elisabeth Roth; Anna Gutowska; Vladimir Kasyanov; Carol Eisenberg; Adrian Neagu; Gabor Forgacs; Roger R. Markwald

Organ printing: Computer-aided jet printer-based three-dimensional tissue engineering

Vladimir Mironov, Thomas Boland, Chris Wilson, Elisabeth Roth, Anna Gutowska, Vladimir Kasyanov, Carol Eisenberg, Adrian Neagu, Gabor Forgacs, Roger R. Markwald

Institute of Anatomy and Anthropology

Research output: Contribution to conference › Paper › peer-review

1 Citation (Scopus)

Abstract

The development of rapid prototyping technologies including the "printing" of biodegradable scaffolds is a fasten growing field of tissue engineering. Here, we report about the development a novel computer-aided jet-based printer for organ printing. This cost effective, high throughput printer was shown to be very useful for two-dimensional molecular and cellular printing. We also show that it is possible to obtain 3-dimensional tissue constructs using a computer-driven, jet-based cell printer, a nontoxic thermo-reversible gel, cell aggregates of stem cells taken from bone marrow and perfusion bioreactor. It was demonstrated in vitro that closely placed explants of embryonic mesenchymal cushion tissue in three-dimensional collagen type 1 gel can fuse into ring-like and tubular structures. Similarly, printed cell aggregates fused and evolved into 3-dimensional, ring-like structure. Finally, a novel mathematical model of the cell-aggregates fusion process in the three-dimensional gels was developed. Taken together these data demonstrate the feasibility of computer-aided, three-dimensional tissue and organ printing.

Original language	English
Pages	9
Number of pages	1
Publication status	Published - 2002
Event	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, GA, United States Duration: 13 Oct 2002 → 16 Oct 2002

Conference

Conference	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products
Country/Territory	United States
City	Atlanta, GA
Period	13/10/02 → 16/10/02

Field of Science*

2.6 Medical engineering
3.4 Medical biotechnology

Publication Type*

3.3. Publications in conference proceedings indexed in Web of Science and/or Scopus database

Cite this

Mironov, V., Boland, T., Wilson, C., Roth, E., Gutowska, A., Kasyanov, V., Eisenberg, C., Neagu, A., Forgacs, G., & Markwald, R. R. (2002). Organ printing: Computer-aided jet printer-based three-dimensional tissue engineering. 9. Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.

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Mironov, V, Boland, T, Wilson, C, Roth, E, Gutowska, A, Kasyanov, V, Eisenberg, C, Neagu, A, Forgacs, G & Markwald, RR 2002, 'Organ printing: Computer-aided jet printer-based three-dimensional tissue engineering', Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States, 13/10/02 - 16/10/02 pp. 9.

TY - CONF

T1 - Organ printing

T2 - Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products

AU - Mironov, Vladimir

AU - Boland, Thomas

AU - Wilson, Chris

AU - Roth, Elisabeth

AU - Gutowska, Anna

AU - Kasyanov, Vladimir

AU - Eisenberg, Carol

AU - Neagu, Adrian

AU - Forgacs, Gabor

AU - Markwald, Roger R.

PY - 2002

Y1 - 2002

N2 - The development of rapid prototyping technologies including the "printing" of biodegradable scaffolds is a fasten growing field of tissue engineering. Here, we report about the development a novel computer-aided jet-based printer for organ printing. This cost effective, high throughput printer was shown to be very useful for two-dimensional molecular and cellular printing. We also show that it is possible to obtain 3-dimensional tissue constructs using a computer-driven, jet-based cell printer, a nontoxic thermo-reversible gel, cell aggregates of stem cells taken from bone marrow and perfusion bioreactor. It was demonstrated in vitro that closely placed explants of embryonic mesenchymal cushion tissue in three-dimensional collagen type 1 gel can fuse into ring-like and tubular structures. Similarly, printed cell aggregates fused and evolved into 3-dimensional, ring-like structure. Finally, a novel mathematical model of the cell-aggregates fusion process in the three-dimensional gels was developed. Taken together these data demonstrate the feasibility of computer-aided, three-dimensional tissue and organ printing.

AB - The development of rapid prototyping technologies including the "printing" of biodegradable scaffolds is a fasten growing field of tissue engineering. Here, we report about the development a novel computer-aided jet-based printer for organ printing. This cost effective, high throughput printer was shown to be very useful for two-dimensional molecular and cellular printing. We also show that it is possible to obtain 3-dimensional tissue constructs using a computer-driven, jet-based cell printer, a nontoxic thermo-reversible gel, cell aggregates of stem cells taken from bone marrow and perfusion bioreactor. It was demonstrated in vitro that closely placed explants of embryonic mesenchymal cushion tissue in three-dimensional collagen type 1 gel can fuse into ring-like and tubular structures. Similarly, printed cell aggregates fused and evolved into 3-dimensional, ring-like structure. Finally, a novel mathematical model of the cell-aggregates fusion process in the three-dimensional gels was developed. Taken together these data demonstrate the feasibility of computer-aided, three-dimensional tissue and organ printing.

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M3 - Paper

AN - SCOPUS:20844458262

SP - 9

Y2 - 13 October 2002 through 16 October 2002

ER -

Organ printing: Computer-aided jet printer-based three-dimensional tissue engineering

Abstract

Conference

Field of Science*

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