Overexpression of DWARF AND LESION FORMATION 1 (DLE1) causes altered activation of plant defense system in Arabidopsis thaliana

Youichi Kondou, Kosuke Noguchi, Shinsuke Kutsuna, Mika Kawashima, Arata Yoneda, Mio Ishibashi, Shu Muto, Takanari Ichikawa, Miki Nakazawa, Minami Matsui, Katsushi Manabe

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A novel gain-of-function mutant showing dwarfism and bushy inflorescences was isolated from Arabidopsis activation-tagging lines. Transformed plants, in which the gene responsible for the phenotype of the mutant was overexpressed, exhibited phenotypes similar to constitutive systematic acquired resistance (SAR)-like defense responses including simulation of lesions in leaves and a highly activated PATHOGENESIS-RELATED 1 gene. We, therefore, designated the gene as DWARF AND LESION FORMATION 1 (DLE1) and showed it encodes a DUF 247 protein. Microarray and gene ontology analyses revealed that defense response genes, especially to biotic stimulus, were enriched in DLE1 overexpressing plants. Fusion proteins of the N-terminal fragment of DLE1 and GFP were likely to be localized in the endoplasmic reticulum (ER) in tobacco BY-2 cells. DLE1 may function in the plant defense system responding to environmental stimuli including biotic stresses in the ER.

Original languageEnglish
Pages (from-to)385-392
Number of pages8
JournalPlant Biotechnology
Volume30
Issue number4
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • ER
  • HR
  • PR genes

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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