PAX7, PAX9 and RYK expression in cleft affected tissue

Research output: Contribution to conferenceAbstractpeer-review


Aim. Craniofacial clefts are one of the most common types of congenital malformations. Transcription factors paired box 7 and 9 (PAX7, PAX9) and receptor-like tyrosine kinase (RYK) have been previously associated with the formation of orofacial clefts but their exact involvement and interactions in cleft-affected tissue remains uncertain. This study analyses the presence of PAX7, PAX9 and RYK immunopositive cells within different cleft affected tissue to assess their possible involvement in cleft morphopathogenesis.
Methods. The cleft affected tissue was collected from non-syndromic orofacial cleft patients during cleft correcting surgery (36 patients with unilateral cleft lip, 13 patients with bilateral cleft lip, 26 patients with cleft palate). Control group tissue was obtained from 7 patients without cleft lip and palate. To evaluate the number of immunopositive structures in the cleft affected tissue and the control group a semiquantitative counting method was used. Non-parametric statistical methods (Kruskal-Wallis H test, Mann–Whitney U test, and Spearman’s rank correlation) were used.
Results. Statistically significant differences for the number of PAX7, PAX9 and RYK positive cells were notified between the controls and the patient groups. Multiple statistically significant correlations between the factors were found in each cleft affected tissue group.
Conclusions. PAX7, PAX9 and RYK have a variable involvement and interaction in postnatal morphopathogenesis of orofacial clefts. PAX7 is more associated with the formation of unilateral cleft lip, while PAX9 relates more towards the isolated cleft palate. The stable presence of RYK in all cleft types indicates its possible participation in different facial cleft formation.
Original languageEnglish
Number of pages1
Publication statusPublished - 4 Jun 2022
Event10th Congress of Baltic Association for Maxillofacial and Plastic Surgery and 16th Joint Symposium Riga-Rostock: Emerging Technologies in Oral and Maxillofacial Surgery - Krogus iela 1, Zemgales priekšpilsēta, Rīga, LV-1048, Rīga, Latvia
Duration: 3 Jun 20224 Jun 2022
Conference number: 10


Congress10th Congress of Baltic Association for Maxillofacial and Plastic Surgery and 16th Joint Symposium Riga-Rostock
Abbreviated titleBAMPS 2022
Other10th BAMPS Congress and16th Joint Symposium Riga-Rostock
Internet address

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 3.4. Other publications in conference proceedings (including local)


Dive into the research topics of 'PAX7, PAX9 and RYK expression in cleft affected tissue'. Together they form a unique fingerprint.

Cite this