Perfusion Bioreactor for Vascular Tissue Engineering with Capacities for Longitudinal Stretch

Vladimir Mironov, Vladimir Kasyanov, Kelly McAllister, Sherrell Oliver, Joe Sistino, Roger Markwald

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Arterial growth during embryonic vascular development is associated with longitudinal strain. The longitudinal strain is an important element of the embryonic vascular mechanical environment (EVME). Thus, a perfusion bioreactor for vascular tissue engineered constructs must include the functional capacity for longitudinal strain. To accomplish this goal, a perfusion bioreactor with the capacity for longitudinal strain was developed. The bioreactor includes two media perfusion systems: one for the inside perfusion and one for the outside perfusion of the cardiovascular engineered tubular construct (CETC). The watertight perfusion chamber allows periodic changing of longitudinal strain of the construct during mechanical conditioning. The range of the longitudinal strain is 0% to 200%. The biomechanical properties of the CETC are controlled by a pressure transducer and a digital TV camera. The pressure transducer and TV camera are connected to a computer. This allows the recording of a relationship between the radius of the VTEG and pressure in both static and dynamic regimens. This bioreactor can perform biomechanical conditioning with longitudinal strain.

Original languageEnglish
Pages (from-to)340-347
Number of pages8
JournalJournal of Craniofacial Surgery
Volume14
Issue number3
DOIs
Publication statusPublished - May 2003
Event
10th International Meeting of the International-Study-Group-for-Tryptophan-Research (ISTRY)
- Padova, Italy
Duration: 25 Jun 200229 Jun 2002

Keywords*

  • Bioreactor
  • Longitudinal strain
  • Mechanical conditioning
  • Tissue engineering

Field of Science*

  • 3.2 Clinical medicine

Publication Type*

  • 3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database

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