TY - JOUR
T1 - Plasma and urinary extracellular vesicles as a source of RNA biomarkers for prostate cancer in liquid biopsies
AU - Bajo-Santos, Cristina
AU - Brokāne, Agnese
AU - Zayakin, Pawel
AU - Endzeliņš, Edgars
AU - Soboļevska, Kristīne
AU - Belovs, Alberts
AU - Jansons, Juris
AU - Sperga, Māris
AU - Llorente, Alicia
AU - Radoviča-Spalviņa, Ilze
AU - Lietuvietis, Vilnis
AU - Linē, Aija
N1 - Publisher Copyright:
Copyright © 2023 Bajo-Santos, Brokāne, Zayakin, Endzeliņš, Soboļevska, Belovs, Jansons, Sperga, Llorente, Radoviča-Spalviņa, Lietuvietis and Linē.
PY - 2023/2/3
Y1 - 2023/2/3
N2 - Introduction: Extracellular vesicles (EVs) have emerged as a very attractive source of cancer- derived RNA biomarkers for the early detection, prognosis and monitoring of various cancers, including prostate cancer (PC). However, biofluids contain a mixture of EVs released from a variety of tissues and the fraction of total EVs that are derived from PC tissue is not known. Moreover, the optimal biofluid—plasma or urine—that is more suitable for the detection of EV- enclosed RNA biomarkers is not yet clear. Methodology: In the current study, we performed RNA sequencing analysis of plasma and urinary EVs collected before and after radical prostatectomy, and matched tumor and normal prostate tissues of 10 patients with prostate cancer. Results and Discussion: The most abundant RNA biotypes in EVs were miRNA, piRNA, tRNA, lncRNA, rRNA and mRNA. To identify putative cancer-derived RNA biomarkers, we searched for RNAs that were overexpressed in tumor as compared to normal tissues, present in the pre-operation EVs and decreased in the post-operation EVs in each RNA biotype. The levels of 63 mRNAs, 3 lncRNAs, 2 miRNAs and 1 piRNA were significantly increased in the tumors and decreased in the post-operation urinary EVs, thus suggesting that these RNAs mainly originate from PC tissue. No such RNA biomarkers were identified in plasma EVs. This suggests that the fraction of PC-derived EVs in urine is larger than in plasma and allows the detection and tracking of PC-derived RNAs.
AB - Introduction: Extracellular vesicles (EVs) have emerged as a very attractive source of cancer- derived RNA biomarkers for the early detection, prognosis and monitoring of various cancers, including prostate cancer (PC). However, biofluids contain a mixture of EVs released from a variety of tissues and the fraction of total EVs that are derived from PC tissue is not known. Moreover, the optimal biofluid—plasma or urine—that is more suitable for the detection of EV- enclosed RNA biomarkers is not yet clear. Methodology: In the current study, we performed RNA sequencing analysis of plasma and urinary EVs collected before and after radical prostatectomy, and matched tumor and normal prostate tissues of 10 patients with prostate cancer. Results and Discussion: The most abundant RNA biotypes in EVs were miRNA, piRNA, tRNA, lncRNA, rRNA and mRNA. To identify putative cancer-derived RNA biomarkers, we searched for RNAs that were overexpressed in tumor as compared to normal tissues, present in the pre-operation EVs and decreased in the post-operation EVs in each RNA biotype. The levels of 63 mRNAs, 3 lncRNAs, 2 miRNAs and 1 piRNA were significantly increased in the tumors and decreased in the post-operation urinary EVs, thus suggesting that these RNAs mainly originate from PC tissue. No such RNA biomarkers were identified in plasma EVs. This suggests that the fraction of PC-derived EVs in urine is larger than in plasma and allows the detection and tracking of PC-derived RNAs.
KW - digital droplet PCR
KW - extracellular vesicles
KW - liquid biopsy
KW - prostate cancer
KW - RNA biotypes
KW - RNA sequencing
UR - http://www.scopus.com/inward/record.url?scp=85148382421&partnerID=8YFLogxK
U2 - 10.3389/fmolb.2023.980433
DO - 10.3389/fmolb.2023.980433
M3 - Article
AN - SCOPUS:85148382421
SN - 2296-889X
VL - 10
JO - Frontiers in Molecular Biosciences
JF - Frontiers in Molecular Biosciences
M1 - 980433
ER -
