Abstract
Aim. This study aimed to examine the effect of Larifan on metabolic characteristics of human
blood monocytes and granulocytes in vitro.
Methods. Four healthy adult men aged 21–26 years were recruited to participate in the study as
blood donors. The metabolic profile of human blood monocytes and granulocytes was evaluated by
phagocytic activity, reactive oxygen species production, nitric oxide generation, and arginase activity. Phagocytosis of FITC-labeled inactivated Staphylococcus aureus and reactive oxygen species
generation were estimated by flow cytometry. Arginase activity was assessed in cell lysates, and
nitric oxide generation in supernatants was examined using the Griess reaction.
Results. Phagocytic index and reactive oxygen species generation were found to be lower in both
human blood monocytes and granulocytes treated with Larifan. The drug caused a dose-dependent
increase in nitric oxide production, as well as a decrease in the arginase activity of blood monocytes.
Conclusions. Our results indicate the ability of Larifan to reinforce the antiviral properties of
resting phagocytes along with containment of oxidative stress development
blood monocytes and granulocytes in vitro.
Methods. Four healthy adult men aged 21–26 years were recruited to participate in the study as
blood donors. The metabolic profile of human blood monocytes and granulocytes was evaluated by
phagocytic activity, reactive oxygen species production, nitric oxide generation, and arginase activity. Phagocytosis of FITC-labeled inactivated Staphylococcus aureus and reactive oxygen species
generation were estimated by flow cytometry. Arginase activity was assessed in cell lysates, and
nitric oxide generation in supernatants was examined using the Griess reaction.
Results. Phagocytic index and reactive oxygen species generation were found to be lower in both
human blood monocytes and granulocytes treated with Larifan. The drug caused a dose-dependent
increase in nitric oxide production, as well as a decrease in the arginase activity of blood monocytes.
Conclusions. Our results indicate the ability of Larifan to reinforce the antiviral properties of
resting phagocytes along with containment of oxidative stress development
Original language | English |
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Pages (from-to) | 69-75 |
Journal | Biotechnologia Acta |
Volume | 16 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2023 |
Externally published | Yes |
Keywords*
- monocytes
- granulocytes
- phagocytosis
- reactive oxygen species
- nitric oxide
- arginase
- metabolic polarization
Field of Science*
- 1.6 Biological sciences
- 3.1 Basic medicine
Publication Type*
- 1.4. Reviewed scientific article published in Latvia or abroad in a scientific journal with an editorial board (including university editions)