Abstract
Growth and development of human foetus is determined by the status of maternal-foetal unit; processes in this unit, having impact on the foetal well-being and outcome of pregnancy, are not always clinically evident. Understanding of molecular processes in placenta, maternal-foetal interface, can possibly unhide those processes and witness presence of undetected events, possibly causing unexpected
outcome.
Hox genes are known to play a significant role in the establishment of pregnancy, maintenance and development of trophoblastic cells and development of human embryo; gestation dependent variations have not been established. The aim of the study was to assess presence of HoxB3 gene products in cells of placentas of different gestational ages and look for correlations with the gestational age and maternal and neonatal anthropometrical parameters.
Samples from different parts of placenta were taken immediately after delivery, placed into picric acid-formaldehyde fixation and brought to the Institute of Anatomy and Anthropology of Rīga Stradiņš
University. Samples were prepared and processed with polyclonal rabbit antibody, manufactured by Santa Cruz, in the working dilution of 1 : 100. Amount of HoxB3 positive cells was detected visually
and ranked from 0 to 4 (abundance). As the distribution was close to normal, Pearson correlation was used for statistical evaluation, significance determined at p < 0.05. HoxB3 positive cells were found in all the samples with no correlations with the gestational age. In pre-term placentas appeared a negative correlation of HoxB3 positive cells with ponderal index of the neonate, and in term placentas positive correlation with the maternal body height. We concluded that HoxB3 gene has an impact on longitudinal
growth of foetus throughout the second and the third trimesters of pregnancy.
outcome.
Hox genes are known to play a significant role in the establishment of pregnancy, maintenance and development of trophoblastic cells and development of human embryo; gestation dependent variations have not been established. The aim of the study was to assess presence of HoxB3 gene products in cells of placentas of different gestational ages and look for correlations with the gestational age and maternal and neonatal anthropometrical parameters.
Samples from different parts of placenta were taken immediately after delivery, placed into picric acid-formaldehyde fixation and brought to the Institute of Anatomy and Anthropology of Rīga Stradiņš
University. Samples were prepared and processed with polyclonal rabbit antibody, manufactured by Santa Cruz, in the working dilution of 1 : 100. Amount of HoxB3 positive cells was detected visually
and ranked from 0 to 4 (abundance). As the distribution was close to normal, Pearson correlation was used for statistical evaluation, significance determined at p < 0.05. HoxB3 positive cells were found in all the samples with no correlations with the gestational age. In pre-term placentas appeared a negative correlation of HoxB3 positive cells with ponderal index of the neonate, and in term placentas positive correlation with the maternal body height. We concluded that HoxB3 gene has an impact on longitudinal
growth of foetus throughout the second and the third trimesters of pregnancy.
| Original language | English |
|---|---|
| Title of host publication | Rīga Stradiņš University Collection of Scientific Papers 2013 |
| Subtitle of host publication | Research articles in medicine & pharmacy |
| Place of Publication | Rīga |
| Publisher | Rīgas Stradiņa universitāte |
| Pages | 91-98 |
| ISBN (Print) | 978-9984-793-43-6 |
| Publication status | Published - 2014 |
Publication series
| Name | Collection of Scientific Papers |
|---|---|
| Publisher | Rīga Stradiņš University |
| ISSN (Print) | 1691-497X |
Keywords*
- placenta
- immunohistochemistry
- HoxB3 products
Field of Science*
- 3.1 Basic medicine
- 3.2 Clinical medicine
Publication Type*
- 3.2. Articles or chapters in other proceedings other than those included in 3.1., with an ISBN or ISSN code