TY - JOUR
T1 - Prolonged Endurance Exercise Increases Macrophage Content and Mitochondrial Respiration in Adipose Tissue in Trained Men
AU - Sahl, Ronni Eg
AU - Patsi, Ioanna
AU - Hansen, Mikkel Thunestvedt
AU - Rømer, Tue
AU - Frandsen, Jacob
AU - Rasmusen, Hanne Kruuse
AU - Ingersen, Arthur
AU - Poulsen, Steen Seier
AU - Dela, Flemming
AU - Larsen, Steen
AU - Helge, Jørn Wulff
N1 - Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved.
PY - 2024/2/1
Y1 - 2024/2/1
N2 - Background: The aim of this study was to investigate the effect of prolonged endurance exercise on adipose tissue inflammation markers and mitochondrial respiration in younger and older men. Methods: "Young"(aged 30 years, n = 7) and "old"(aged 65 years, n = 7) trained men were exposed to an exercise intervention of 15 consecutive days biking 7 to 9 hours/day at 63% and 65% of maximal heart rate (young and old, respectively), going from Copenhagen, Denmark to Palermo, Italy. Adipose tissue was sampled from both the gluteal and abdominal depot before and after the intervention. Mitochondrial respiration was measured by high-resolution respirometry, and adipose inflammation was assessed by immunohistochemical staining of paraffin embedded sections. Results: An increased number of CD163+ macrophages was observed in both the gluteal and abdominal depot (P <. 01). In addition, an increased mitochondrial respiration was observed in the abdominal adipose tissue from men in the young group with complex I (CIp) stimulated respiration, complex I + II (CI+IIp) stimulated respiration and the capacity of the electron transport system (ETS) (P <. 05), and in the older group an increase in CIp and CI+IIp stimulated respiration (P <. 05) was found. Conclusion: Overall, we found a positive effect of prolonged endurance exercise on adipose tissue inflammation markers and mitochondrial respiration in both young and old trained men, and no sign of attenuated function in adipose tissue with age.
AB - Background: The aim of this study was to investigate the effect of prolonged endurance exercise on adipose tissue inflammation markers and mitochondrial respiration in younger and older men. Methods: "Young"(aged 30 years, n = 7) and "old"(aged 65 years, n = 7) trained men were exposed to an exercise intervention of 15 consecutive days biking 7 to 9 hours/day at 63% and 65% of maximal heart rate (young and old, respectively), going from Copenhagen, Denmark to Palermo, Italy. Adipose tissue was sampled from both the gluteal and abdominal depot before and after the intervention. Mitochondrial respiration was measured by high-resolution respirometry, and adipose inflammation was assessed by immunohistochemical staining of paraffin embedded sections. Results: An increased number of CD163+ macrophages was observed in both the gluteal and abdominal depot (P <. 01). In addition, an increased mitochondrial respiration was observed in the abdominal adipose tissue from men in the young group with complex I (CIp) stimulated respiration, complex I + II (CI+IIp) stimulated respiration and the capacity of the electron transport system (ETS) (P <. 05), and in the older group an increase in CIp and CI+IIp stimulated respiration (P <. 05) was found. Conclusion: Overall, we found a positive effect of prolonged endurance exercise on adipose tissue inflammation markers and mitochondrial respiration in both young and old trained men, and no sign of attenuated function in adipose tissue with age.
KW - adipose tissue
KW - aging
KW - exercise
KW - human
KW - inflammation
KW - mitochondrial respiration
UR - http://www.scopus.com/inward/record.url?scp=85182957070&partnerID=8YFLogxK
U2 - 10.1210/clinem/dgad509
DO - 10.1210/clinem/dgad509
M3 - Article
C2 - 37643899
AN - SCOPUS:85182957070
SN - 0021-972X
VL - 109
SP - e799-e808
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 2
ER -
