Quantitative analysis of phenibut in rat brain tissue extracts by liquid chromatography-tandem mass spectrometry

Solveiga Grinberga, Liga Zvejniece, Edgars Liepinsh, Maija Dambrova, Osvalds Pugovics

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Phenibut (3-phenyl-4-aminobutyric acid) is a γ-aminobutyric acid mimetic drug, which is used clinically as a mood elevator and tranquilizer. In the present work, a rapid, selective and sensitive liquid chromatography-tandem mass spectrometry method for quantification of phenibut in biological matrices has been developed. The method is based on protein precipitation with acidic acetonitrile followed by isocratic chromatographic separation using acetonitrile-formic acid (0.1% in water; 8:92, v/v) mobile phase on a reversed-phase column. Detection of the analyte was performed by electrospray ionization mass spectrometry in multiple reaction monitoring mode with the precursor-to-product ion transition m/z 180.3 → m/z 117.2. The calibration curve was linear over the concentration range 50-2000 ng/mL. The lower limit of quantification for phenibut in rat brain extracts was 50 ng/mL. Acceptable precision and accuracy were obtained over the whole concentration range. The validated method was successfully applied in a pharmacological study to analyze phenibut concentration in rat brain tissue extract samples.

Original languageEnglish
Pages (from-to)1321-1324
Number of pages4
JournalBiomedical Chromatography
Volume22
Issue number12
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • Brain tissue extracts
  • LC-MS-MS
  • Phenibut

Field of Science

  • 3.1 Basic medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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