Quantitative changes of bone volume and immunohistochemical analysis of biomarkers in healthy, osteoporotic and osteoporotic sham surgery affected rabbit bone controls

Aim of our preliminary in vivo study was to evaluate bone regeneration properties in three different bone conditions and to compare expression of OPG, NFkB105, BMP2/4, MMP2, IL 1 and IL 10 between healthy, osteoporotic and osteoporotic sham surgery affected rabbit bone controls. Osteoporosis index was found to be higher in healthy bone, while rabbits with osteoporotic sham surgery affected bone showed slight elevation of mean trabecular field rather than rabbits with osteoporotic bone. Expression of OPG and NFkB105 was higher in healthy bone, with less equal appearance in osteoporotic and osteoporotic sham surgery affected bone. Presence of BMP2/4 and MMP2 was much higher in healthy bone, whereas twice less in osteoporotic group. Osteoporotic sham surgery affected group showed recurrence of immunoreactive structures similar to healthy group of IL 1 and IL 10. OPG and was found as the most stable indicators for bone regeneration. Decrease of BMP2/4, MMP2, IL-1 and 1L-10 in osteoporotic bone with following increase in osteoporotic sham surgery affected bone, proves the role of traumatic injury as the inducer of bone regeneration.