Quantitative Immunohistochemical Analysis of Osteoporotic Bone of Experimental Animals in New Biphasic Calcium Phosphate Bioceramic Implantation Area

Sandris Petronis, Māra Pilmane, Andrejs Skaģers, Vita Zalite

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

To obtain detailed information on osteoporotic bone regeneration and remodelling as a model for the use of experimental animals with induced osteoporosis, a similar morphology as osteoporotic human bone was chosen.
Immunohistochemical analysis of experimental animal osteoporotic bone 3 months after implantation of calcium phosphate bioceramic granules was performed.
Experimental osteoporosis was induced in 7 female rabbits, aged 2 to 2.8 years, after ovariectomy followed by administration of 1 mg/kg of methylprednisolone daily for 8 weeks. In the experimental group of 4 animals, bone defect in hip bone was created and filled with a biphasic calcium phosphate bioceramic granules with a size of 1 to 1.4 mm, containing hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) in proportion of 90/10 developed in RTU Rudolfs Cimdins Rīga Biomaterials Innovation and Development Centre. The control group had 3 rabbits without bioceramics implantation. After 3 months, all of the animals were euthanized and bone samples collected and prepared to analyse following immunohistochemistry markers: osteocalcin (OC), osteopontin (OP), bone morphogenetic protein 2/4 (BMP2/4), transforming growth factor beta (TGFβ). Semiquantitative method was used in result evaluation.
The smallest difference in experimental and control groups was in TGFβ expression. Osteocalcin expression in the investigated samples is relatively more pronounced, indicating the ability of bioceramics to improve bone quality. Investigated samples of the experimental group OP positive cells compared to OC positive cells is significantly lower, which may indicate bone remodelling, in which osteoblast function exceeds osteoclast activity. In our study, OPG expression in bone around the HAp/TCP implantation sites is twice as high as in the control group without bioceramic implantation, suggesting osteogenesis dominance over bone resorption.
HAp/TCP bioceramic implants in osteoporotic bone have a positive effect on bone quality and promotes bone remodelling towards new bone formation.
Translated title of the contributionQuantitative Immunohistochemical Analysis of Osteoporotic Bone of Experimental Animals in New Biphasic Calcium Phosphate Bioceramic Implantation Area
Original languageLatvian
Title of host publicationRīgas Stradiņa universitātes Zinātniskie raksti 
Subtitle of host publication2014. gada medicīnas nozares pētnieciskā darba publikācijas
Place of PublicationRīga
PublisherRīgas Stradiņa universitāte
Pages262-268
ISBN (Print)978-9984-793-68-9
Publication statusPublished - 2015

Publication series

NameRSU Zinātniskie Raksti
ISSN (Print)1407-9453

Keywords*

  • experimental osteoporosis
  • calcium phosphate bioceramics
  • immunohistochemistry

Field of Science*

  • 3.1 Basic medicine
  • 3.2 Clinical medicine

Publication Type*

  • 3.2. Articles or chapters in other proceedings other than those included in 3.1., with an ISBN or ISSN code

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