Rapid biofabrication of tubular tissue constructs by centrifugal casting in a decellularized natural scaffold with laser-machined micropores

Vladimir A. Kasyanov, Jason Hodde, Michael C. Hiles, Carol Eisenberg, Leonard Eisenberg, Luis E.F. De Castro, Iveta Ozolanta, Modra Murovska, Robert A. Draughn, Glenn D. Prestwich, Roger R. Markwald, Vladimir Mironov

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Centrifugal casting allows rapid biofabrication of tubular tissue constructs by suspending living cells in an in situ cross-linkable hydrogel. We hypothesize that introduction of laser-machined micropores into a decellularized natural scaffold will facilitate cell seeding by centrifugal casting and increase hydrogel retention, without compromising the biomechanical properties of the scaffold. Micropores with diameters of 50, 100, and 200 μm were machined at different linear densities in decellularized small intestine submucosa (SIS) planar sheets and tubular SIS scaffolds using an argon laser. The ultimate stress and ultimate strain values for SIS sheets with laser-machined micropores with diameter 50 μm and distance between holes as low as 714 μm were not significantly different from unmachined control SIS specimens. Centrifugal casting of GFP-labeled cells suspended in an in situ cross-linkable hyaluronan-based hydrogel resulted in scaffold recellularization with a high density of viable cells inside the laser-machined micropores. Perfusion tests demonstrated the retention of the cells encapsulated within the HA hydrogel in the microholes. Thus, an SIS scaffold with appropriately sized microholes can be loaded with hydrogel encapsulated cells by centrifugal casting to give a mechanically robust construct that retains the cell-seeded hydrogel, permitting rapid biofabrication of tubular tissue construct in a "bioreactor-free" fashion.

Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalJournal of Materials Science: Materials in Medicine
Volume20
Issue number1
DOIs
Publication statusPublished - Jan 2009

Field of Science

  • 2.6 Medical engineering
  • 2.5 Materials engineering
  • 3.4 Medical biotechnology

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

Fingerprint

Dive into the research topics of 'Rapid biofabrication of tubular tissue constructs by centrifugal casting in a decellularized natural scaffold with laser-machined micropores'. Together they form a unique fingerprint.

Cite this