Rapid Mapping of NotI Linking Clones with Differential Hybridization and Alu-PCR

Eugene R. Zabarovsky, Vladimir I. Kashuba, Irina D. Kholodnyuk, Veronika I. Zabarovska, Eric J. Stanbridge, George Klein

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

For construction of a NotI restriction map of the human genome, the isolation and mapping of unique NotI linking clones represent important and critical steps. Recently we have shown that an Alu-PCR approach can be used for isolation of NotI linking clones from defined regions of the chromosomes. This represents a useful method for isolating and analyzing a small number of clones, but it would be laborious to use it for mapping many Not I linking clones simultaneously. Here we suggest another modification of Alu-PCR for rapid concurrent mapping of many NotI linking clones. The results clearly demonstrate the utility of this approach. Seventy-one random NotI linking clones were analyzed. Among them, 65 clones (91.5%) were correctly selected and mapped using this approach. With differential hybridization and Alu-PCR, a significant portion of all human NotI linking clones (=30%) can be rapidly mapped to particular chromosomes or to defined regions of these chromosomes.

Original languageEnglish
Pages (from-to)486-489
Number of pages4
JournalGenomics
Volume21
Issue number3
DOIs
Publication statusPublished - Jun 1994
Externally publishedYes

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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