Recapitulation of transcriptomic characteristics of primary breast tumours in patient-derived 3D cultures in vitro

Dina Nitiša, Nityanand Jain, Arvīds Irmejs, Valdis Pirsko, Inese Čakstiņa (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

Abstract

Breast cancer (BC) is the most common cause of cancer-related deaths among women in Europe and worldwide. Adherent (2D) cell cultures have been the routine in vitro model system employed in preclinical BC research for the last half-century. Over the past decade, new protocols have been developed allowing patient-derived three-dimensional organoid (3D) cell culture development from a range of solid tumours, including BC. These 3D models offer a promise of closer resemblance to the native tumour than the 2D cultures. To test the assumption that an in vitro 3D BC model system provides increased faithfulness to the molecular processes happening in vivo, as compared to 2D BC cultures, post-operational material from three BC patients was used to simultaneously develop 2D and 3D cultures in vitro. When analysed by quantitative polymerase chain reaction (PCR), the gene expression patterns of the cells from 3D cultures resembled the original tissues, while the gene expression patterns of the conventional 2D cultures were more distant.

Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalProceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
Volume75
Issue number1
DOIs
Publication statusPublished - 1 Feb 2021

Keywords

  • 3D culture
  • Organoids
  • Patient-derived cell culture
  • Primary breast cancer
  • QPCR

Field of Science

  • 3.2 Clinical medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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