Reliability and variation in mitochondrial respiration in human adipose tissue

Ronni Eg Sahl; Eva Frederikke Høy Helms; Malte Schmücker; Mathias Flensted-Jensen; Arthur Ingersen; Thomas Morville; Flemming Dela; Jørn Wulff Helge; Steen Larsen

doi:10.1080/21623945.2021.1991617

Reliability and variation in mitochondrial respiration in human adipose tissue

Ronni Eg Sahl (Corresponding Author)
, Eva Frederikke Høy Helms
, Malte Schmücker
, Mathias Flensted-Jensen
, Arthur Ingersen
, Thomas Morville
, Flemming Dela
, Jørn Wulff Helge
, Steen Larsen

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

2 Downloads (Pure)

Abstract

Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.

Original language	English
Pages (from-to)	605-611
Number of pages	7
Journal	Adipocyte
Volume	10
Issue number	1
DOIs	https://doi.org/10.1080/21623945.2021.1991617
Publication status	Published - 2021
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords*

Adipose tissue
coefficient of variation
high resolution respirometry
human
mitochondria

Field of Science*

3.1 Basic medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1080/21623945.2021.1991617Licence: CC BY

Reliability and variation in mitochondrial respirationFinal published version, 1 MBLicence: CC BY

Cite this

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title = "Reliability and variation in mitochondrial respiration in human adipose tissue",

abstract = "Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.",

keywords = "Adipose tissue, coefficient of variation, high resolution respirometry, human, mitochondria",

author = "Sahl, \{Ronni Eg\} and \{H{\o}y Helms\}, \{Eva Frederikke\} and Malte Schm{\"u}cker and Mathias Flensted-Jensen and Arthur Ingersen and Thomas Morville and Flemming Dela and Helge, \{J{\o}rn Wulff\} and Steen Larsen",

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year = "2021",

doi = "10.1080/21623945.2021.1991617",

language = "English",

volume = "10",

pages = "605--611",

journal = "Adipocyte",

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TY - JOUR

T1 - Reliability and variation in mitochondrial respiration in human adipose tissue

AU - Sahl, Ronni Eg

AU - Høy Helms, Eva Frederikke

AU - Schmücker, Malte

AU - Flensted-Jensen, Mathias

AU - Ingersen, Arthur

AU - Morville, Thomas

AU - Dela, Flemming

AU - Helge, Jørn Wulff

AU - Larsen, Steen

PY - 2021

Y1 - 2021

N2 - Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.

AB - Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.

KW - Adipose tissue

KW - coefficient of variation

KW - high resolution respirometry

KW - human

KW - mitochondria

UR - https://www.scopus.com/pages/publications/85120176224

U2 - 10.1080/21623945.2021.1991617

DO - 10.1080/21623945.2021.1991617

M3 - Article

AN - SCOPUS:85120176224

SN - 2162-3945

VL - 10

SP - 605

EP - 611

JO - Adipocyte

JF - Adipocyte

IS - 1

ER -

Reliability and variation in mitochondrial respiration in human adipose tissue

Abstract

UN SDGs

Keywords*

Field of Science*

Publication Type*

Access to Document

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