Replication study of ulcerative colitis risk loci in a Lithuanian-Latvian case control sample

Jurgita Skieceviciene, Gediminas Kiudelis, Eva Ellinghaus, Tobias Balschun, Laimas V. Jonaitis, Aida Zvirbliene, Goda Denapiene, Marcis Leja, Gitana Pranculiene, Vytenis Kalibatas, Hamidreza Saadati, David Ellinghaus, Vibeke Andersen, Jonas Valantinas, Algimantas Irnius, Aleksejs Derovs, Algimantas Tamelis, Stefan Schreiber, Limas Kupcinskas, Andre Franke

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)
17 Downloads (Pure)

Abstract

Background: Differences between populations might be reflected in their different genetic risk maps to complex diseases, for example, inflammatory bowel disease. We here investigated the role of known inflammatory bowel disease associated single nucleotide polymorphisms (SNPs) in a subset of patients with ulcerative colitis (UC) from the Northeastern European countries Lithuania and Latvia and evaluated possible epistatic interactions between these genetic variants. Methods: We investigated 77 SNPs derived from 5 previously published genome-wide association studies for Crohn's disease and UC. Our study panel comprised 444 Lithuanian and Latvian patients with UC and 1154 healthy controls. Single marker case control association and SNP-SNP epistasis analyses were performed. Results: We found 14 SNPs tagging 9 loci, including 21q21.1, NKX2-3, MST1, the HLA region, 1p36.13, IL10, JAK2, ORMDL3, and IL23R, to be associated with UC. Interestingly, the association of UC with previously identified variants in the HLA region was not the strongest association in our study (P = 4.34 × 1023, odds ratio [OR] = 1.25), which is in contrast to all previously published studies. No association with any disease subphenotype was found. SNP-SNP interaction analysis showed significant epistasis between SNPs in the PTPN22 (rs2476601) and C13orf31 (rs3764147) genes and increased risk for UC (P = 1.64 × 1026, OR = 2.44). The association has been confirmed in the Danish study group (P = 0.04, OR = 3.25). Conclusions: We confirmed the association of the 9 loci (21q21.1, 1p36.13, NKX2-3, MST1, the HLA region, IL10, JAK2, ORMDL3, and IL23R) with UC in the Lithuanian Latvian population. SNP-SNP interaction analyses showed that the combination of SNPs in the PTPN22 (rs2476601) and C13orf31 (rs3764147) genes increase the risk for UC.

Original languageEnglish
Pages (from-to)2349-2355
Number of pages7
JournalInflammatory Bowel Diseases
Volume19
Issue number11
DOIs
Publication statusPublished - Oct 2013

Keywords*

  • Case control
  • Lithuanian-Latvian
  • Single nucleotide polymorphisms
  • Ulcerative colitis

Field of Science*

  • 3.2 Clinical medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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