Role of Interferon-Gamma (Ifn-γ) in Immune Response Regulation in HIV-1 and HIV-1 + Mycobacterium Tuberculosis (Tb) Infected Patients

Inga Januškevica, Baiba Rozentale, Elvīra Hagina, Jeļena Eglīte, Tatjana Kolupajeva, Jeļena Storozenko, Ludmila Guseva, Aivars Lejnieks

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this research was to investigate the role of IFN-γ in interaction between IL-10, IL-18, IL-1b, CD4 cell counts and HIV-1 RNA viral load in the development of HIV-1 in patients co-infected with Mycobacterium tuberculosis (TB). The study was conducted by Rīga East Clinical University Hospital with data from the HIV-1 register, in collaboration with the RSU Joint Laboratory of Clinical Immunology and Immunogenetics. 200 HIV-1 infected patients and 184 HIV-1 with TB co-infection patients divided in four groups were included in the study. IFN-γ, IL-10, IL-18, IL-1b levels were measured in serum with commercially enzyme-linked immunosorbent assay (ELISA Vector-Best Corporation, Novosibirsk, Russia). CD4 cell counts were measured by flow Partec IVD cytometry (USA). HIV-1 RNA quantification was performed using the COBAS AmpliPrep/COBAS Taqman HIV-1 Test (Germany). All groups were compared with each another. IFN-γ production was significantly lower, and IL-10 and CD4 cell counts were significantly higher, in HIV-1 patients without TB compared with the other groups. The group with HIV-1 and TB had significantly elevated IL-18 production. HIV patients with primary TB had significantly elevated IFN-γ production and HIV-1 RNA viral load and significantly lower IL-10 production.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalProceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
Volume70
Issue number4
DOIs
Publication statusPublished - 1 Aug 2016

Keywords

  • cytokines
  • HIV-1
  • IFN-γ
  • Mycobacterium tuberculosis

Field of Science

  • 3.1 Basic medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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