Segregation of genomes in polyploid tumour cells following mitotic catastrophe

Jekaterina Erenpreisa; M. Kalejs; F. Ianzini; E. A. Kosmacek; M. A. MacKey; D. Emzinsh; M. S. Cragg; A. Ivanov; T. M. Illidge

doi:10.1016/j.cellbi.2005.10.008

Segregation of genomes in polyploid tumour cells following mitotic catastrophe

Jekaterina Erenpreisa, M. Kalejs, F. Ianzini, E. A. Kosmacek, M. A. MacKey, D. Emzinsh, M. S. Cragg, A. Ivanov, T. M. Illidge

Research output: Contribution to journal › Article › peer-review

95 Citations (Scopus)

Abstract

Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.

Original language	English
Pages (from-to)	1005-1011
Number of pages	7
Journal	Cell Biology International
Volume	29
Issue number	12
DOIs	https://doi.org/10.1016/j.cellbi.2005.10.008
Publication status	Published - Dec 2005
Externally published	Yes

Keywords*

De-polyploidisation
Link to microtubule-organising centre
Mitotic catastrophe
Radial arrangement of genomes
Resuming of mitosis

Field of Science*

1.6 Biological sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1016/j.cellbi.2005.10.008

Cite this

@article{748d241713574c92802f8d475747c72e,

title = "Segregation of genomes in polyploid tumour cells following mitotic catastrophe",

abstract = "Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.",

keywords = "De-polyploidisation, Link to microtubule-organising centre, Mitotic catastrophe, Radial arrangement of genomes, Resuming of mitosis",

author = "Jekaterina Erenpreisa and M. Kalejs and F. Ianzini and Kosmacek, {E. A.} and MacKey, {M. A.} and D. Emzinsh and Cragg, {M. S.} and A. Ivanov and Illidge, {T. M.}",

note = "Funding Information: We are indebted to Mr Roger Alston, Mrs Sue Cox, Mr Nick Barnet and Dr. Anton Page (Southampton University Imaging Unit) for their many years of assistance in the Southampton University. We are similarly indebted to Mr. Vladislav Zelchs (Biomedicine Centre of the Latvian University) for assistance in electron microscopy; to Drs. Bodo Liebe (Max-Planck-Inst. for Molecular Genetics, Berlin) and Harry Scherthan (Inst. of Radiation Biology, Munich) for their help with FISH, and the latter, in addition, for the helpful discussion of our results. Thanks also go to Dr. D. Emzinsh and Mrs. Galina Boka (Latvian Oncology Centre) for their help in cell irradiation. This work was partly supported by the Latvian Scientific Council, a Grant from the Royal Society of London and a Latvian-USA Governmental Grant enabling exchange visits between Riga and Southampton and Riga and Iowa City, and by the National Institutes of Health Grants CA74899, CA58648, and CA/GM94801, and by the Whitaker Foundation Special Opportunity Award. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2005",

month = dec,

doi = "10.1016/j.cellbi.2005.10.008",

language = "English",

volume = "29",

pages = "1005--1011",

journal = "Cell Biology International",

issn = "1065-6995",

publisher = "Wiley-Blackwell",

number = "12",

}

TY - JOUR

T1 - Segregation of genomes in polyploid tumour cells following mitotic catastrophe

AU - Erenpreisa, Jekaterina

AU - Kalejs, M.

AU - Ianzini, F.

AU - Kosmacek, E. A.

AU - MacKey, M. A.

AU - Emzinsh, D.

AU - Cragg, M. S.

AU - Ivanov, A.

AU - Illidge, T. M.

N1 - Funding Information: We are indebted to Mr Roger Alston, Mrs Sue Cox, Mr Nick Barnet and Dr. Anton Page (Southampton University Imaging Unit) for their many years of assistance in the Southampton University. We are similarly indebted to Mr. Vladislav Zelchs (Biomedicine Centre of the Latvian University) for assistance in electron microscopy; to Drs. Bodo Liebe (Max-Planck-Inst. for Molecular Genetics, Berlin) and Harry Scherthan (Inst. of Radiation Biology, Munich) for their help with FISH, and the latter, in addition, for the helpful discussion of our results. Thanks also go to Dr. D. Emzinsh and Mrs. Galina Boka (Latvian Oncology Centre) for their help in cell irradiation. This work was partly supported by the Latvian Scientific Council, a Grant from the Royal Society of London and a Latvian-USA Governmental Grant enabling exchange visits between Riga and Southampton and Riga and Iowa City, and by the National Institutes of Health Grants CA74899, CA58648, and CA/GM94801, and by the Whitaker Foundation Special Opportunity Award. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2005/12

Y1 - 2005/12

N2 - Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.

AB - Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.

KW - De-polyploidisation

KW - Link to microtubule-organising centre

KW - Mitotic catastrophe

KW - Radial arrangement of genomes

KW - Resuming of mitosis

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U2 - 10.1016/j.cellbi.2005.10.008

DO - 10.1016/j.cellbi.2005.10.008

M3 - Article

C2 - 16314119

AN - SCOPUS:28444437761

SN - 1065-6995

VL - 29

SP - 1005

EP - 1011

JO - Cell Biology International

JF - Cell Biology International

IS - 12

ER -

Segregation of genomes in polyploid tumour cells following mitotic catastrophe

Abstract

Keywords*

Field of Science*

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