TY - JOUR
T1 - Serodiagnosis of human bocavirus 1 infection among hospitalised children with lower respiratory tract infection in Latvia
AU - Ziemele, Inga
AU - Xu, Man
AU - Vilmane, Anda
AU - Rasa-Dzelzkaleja, Santa
AU - Hedman, Klaus
AU - Söderlund-Venermo, Maria
AU - Gardovska, Dace
AU - Nora-Krukle, Zaiga
AU - Murovska, Modra
N1 - Funding Information:
We thank Lea Hedman for technical advice in serology. This study was supported in part by the Rîga Stradiòð University research project “Epidemiology, pathogenicity of human bocavirus (HBoV) types and their possible connection to lower respiratory tract diseases and acute gastroenteritis in children”, RSU ZP 17/2013, National Research Programme “Biomedicine” project Nr. 5.6.2, the Sigrid Jusélius Foundation, the Life and Health Medical Grant Association, the Jane and Aatos Erkko Foundation, the Medical Society of Finland (FLS), and The Finnish Society of Sciences and Letters.
Publisher Copyright:
© 2019 Inga Ziemele et al., published by Sciendo 2019.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Since its discovery in 2005, human bocavirus 1 (HBoV1) has globally been one of the most common respiratory viruses. It is currently accepted that HBoV1 is a pathogen, causing upper and lower respiratory tract infections (LRTIs) in children. However, due to the prolonged HBoV1 DNA shedding from the upper airways and the subsequent high rate of co-detections with other respiratory viruses, the interpretation of positive polymerase chain reaction results is challenging. The aim of this study was to identify acute HBoV1 infections by the presence of HBoV1-specific IgM and IgG measured by competition enzyme immunoassay, to elucidate the induction of Th1/Th2 cytokines, and to describe the clinical characteristics associated with acute HBoV1 infection in hospitalised children less than five years of age with LRTI. HBoV1 IgM was detected in 19/102 (18.6%) and IgG in 66/102 (64.7%) patients. HBoV1 IgM was most frequently found in patients aged 13 to 24 months. Pneumonia and acute wheezing were the most common clinical diagnoses among HBoV1 IgM positive patients. The seroprevalence of HBoV1-specific IgG increased with age, reaching 85% by the age of five years. INF-γ, IL-4, IL-5, and IL-10 were observed to be higher in patients with acute HBoV1 infection.
AB - Since its discovery in 2005, human bocavirus 1 (HBoV1) has globally been one of the most common respiratory viruses. It is currently accepted that HBoV1 is a pathogen, causing upper and lower respiratory tract infections (LRTIs) in children. However, due to the prolonged HBoV1 DNA shedding from the upper airways and the subsequent high rate of co-detections with other respiratory viruses, the interpretation of positive polymerase chain reaction results is challenging. The aim of this study was to identify acute HBoV1 infections by the presence of HBoV1-specific IgM and IgG measured by competition enzyme immunoassay, to elucidate the induction of Th1/Th2 cytokines, and to describe the clinical characteristics associated with acute HBoV1 infection in hospitalised children less than five years of age with LRTI. HBoV1 IgM was detected in 19/102 (18.6%) and IgG in 66/102 (64.7%) patients. HBoV1 IgM was most frequently found in patients aged 13 to 24 months. Pneumonia and acute wheezing were the most common clinical diagnoses among HBoV1 IgM positive patients. The seroprevalence of HBoV1-specific IgG increased with age, reaching 85% by the age of five years. INF-γ, IL-4, IL-5, and IL-10 were observed to be higher in patients with acute HBoV1 infection.
KW - children
KW - cytokines
KW - human bocavirus
KW - lower respiratory tract infection
KW - serology
UR - http://www.scopus.com/inward/record.url?scp=85069737658&partnerID=8YFLogxK
U2 - 10.2478/prolas-2019-0046
DO - 10.2478/prolas-2019-0046
M3 - Article
AN - SCOPUS:85069737658
SN - 1407-009X
VL - 73
SP - 288
EP - 295
JO - Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
JF - Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
IS - 4
ER -