TY - JOUR
T1 - Significant age- and gender-related variability of main lymphocyte subsets in paediatric patients
T2 - Latvian data
AU - Nikulshin, Sergey
AU - Kundzina, Linda
AU - Tolstikova, Iveta
AU - Gravele, Dagne
AU - Prokofjeva, Tatjana
AU - Gardovska, Dace
N1 - Publisher Copyright:
2018 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Foundation for the Scandinavian Journal of Immunology.
PY - 2018/8
Y1 - 2018/8
N2 - Age- and gender-related variability of main lymphocyte subsets (T, B and NK cell absolute counts and percentages from Ly; T4, T8 and DN cell absolute counts and percentages from lymphocytes and from T cells; T4:T8 and T:B ratios) was studied in a large cohort of paediatric patients (2 days-17 years) at yearly intervals. A total of 4128 six-color TBNK tests performed on BD FACSCanto II flow cytometer were assessed; patients with immune deficiencies and tumours were not included. The study revealed significant age- and gender-related changes in all subsets. Absolute counts of T, B, T4 cells dropped from neonates to adolescents, decrease in T8 and NK cells was milder; relative count of T cells increased with age and that of B cells decreased; T4:T8 ratio went down and T:B ratio grew. Total T, T4 cells and T4:T8 ratio were significantly higher in girls, while T8, NK and DN cells were significantly higher in boys; significantly higher relative and absolute B cell counts in boys appeared in adolescence. We compared our results with reference values for healthy children (Tosato et al, Cytometry A. 2015;87:81); there was a good concordance, except for DN cells. Advantages of using patient cohort instead of healthy children as reference, possibilities for adjusting age- and gender-specific reference ranges and potential international data pooling are discussed.
AB - Age- and gender-related variability of main lymphocyte subsets (T, B and NK cell absolute counts and percentages from Ly; T4, T8 and DN cell absolute counts and percentages from lymphocytes and from T cells; T4:T8 and T:B ratios) was studied in a large cohort of paediatric patients (2 days-17 years) at yearly intervals. A total of 4128 six-color TBNK tests performed on BD FACSCanto II flow cytometer were assessed; patients with immune deficiencies and tumours were not included. The study revealed significant age- and gender-related changes in all subsets. Absolute counts of T, B, T4 cells dropped from neonates to adolescents, decrease in T8 and NK cells was milder; relative count of T cells increased with age and that of B cells decreased; T4:T8 ratio went down and T:B ratio grew. Total T, T4 cells and T4:T8 ratio were significantly higher in girls, while T8, NK and DN cells were significantly higher in boys; significantly higher relative and absolute B cell counts in boys appeared in adolescence. We compared our results with reference values for healthy children (Tosato et al, Cytometry A. 2015;87:81); there was a good concordance, except for DN cells. Advantages of using patient cohort instead of healthy children as reference, possibilities for adjusting age- and gender-specific reference ranges and potential international data pooling are discussed.
UR - http://www.scopus.com/inward/record.url?scp=85050290434&partnerID=8YFLogxK
U2 - 10.1111/sji.12696
DO - 10.1111/sji.12696
M3 - Article
AN - SCOPUS:85050290434
SN - 0300-9475
VL - 88
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
IS - 2
M1 - e12696
ER -