Simultaneous identification of amorphous calcium phosphate and S.epidermidis bacteria by photoacoustic spectroscopy

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

The incorporation of biomaterials in human tissue requires methods to study the interface of the implant with the biological setting. We set out to study whether Photoacoustic spectroscopy with a higher level of sensitivity from the cantilever detector could simultaneously detect amorphous calcium phosphate and the bacteria. The calcium phosphate was synthesized, pressed into tablets, and then immersed in a solution containing S.epidermidis bacteria. Spectra were recorded after 1, 2, 3, 4 and 5 days. Deconvolution of the spectra at different time periods was able to separate bands belonging to the bacteria and carbonate bands arising from the calcium phosphate. This allowed the simultaneous identification of the biomaterial and bacteria. It was found that the PAS spectra could not identify the bacterial adhesion process due to the low concentration, but the amide peaks at 3 days inferred colonization of bacteria. This was confirmed by SEM that shoed an increase in the bacteria concentration. This is the first step in showing the simultaneous detection of calcium phosphate and bacteria by Photoacoustic spectroscopy, a method that required more research to show changes on the surface of the implant.

Original languageEnglish
Title of host publicationMultifunctional Bioceramics for Innovative Therapy
EditorsAhmed El-Ghannam
PublisherTrans Tech Publications Ltd
Pages125-129
Number of pages5
ISBN (Print)9783038357568
DOIs
Publication statusPublished - 2017
Event28th Annual Meeting of the International Society for Ceramics in Medicine, Bioceramics 2016 - Charlotte, United States
Duration: 18 Oct 201621 Oct 2016
Conference number: 28

Publication series

NameKey Engineering Materials
Volume720
ISSN (Print)1013-9826
ISSN (Electronic)1662-9795

Conference

Conference28th Annual Meeting of the International Society for Ceramics in Medicine, Bioceramics 2016
Country/TerritoryUnited States
CityCharlotte
Period18/10/1621/10/16

Keywords

  • Amorphous carbonated calcium phosphate (ACCP)
  • Photoacoustic (PA) spectroscopy
  • Scanning electron microscopy (SEM)
  • Surface

Field of Science

  • 1.3 Physical sciences
  • 1.6 Biological sciences
  • 2.5 Materials emgineering

Publication Type

  • 3.3. Publications in conference proceedings indexed in Web of Science and/or Scopus database

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