Tannic acid mimicking dendrimers as small intestine submucosa stabilizing nanomordants

Vladimir Kasyanov, Jason Isenburg, Robert A. Draughn, Starr Hazard, Jason Hodde, Iveta Ozolanta, Modra Murovska, S. Bart Halkes, Ioannis Vrasidas, Rob M.J. Liskamp, Roland J. Pieters, Dan Simionescu, Roger R. Markwald, Vladimir Mironov

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Chemical stabilization resulting in increased resistance to proteolytic degradation is one of the approaches in prevention of post-implantational aneurysm development in decellularized natural vascular scaffolds. Recently, tannic acid (TA) and tannic acid mimicking dendrimers (TAMD) have been suggested as potential stabilization agents for collagen and elastin. The aim of this work was to determine the stabilizing effects of TAMD on decellularized natural scaffolds. Vascular scaffolds fabricated from small intestine submucosa (SIS) and SIS plane sheets (Cook Biotech Inc.) were used. The biomechanical properties of the SIS vascular graft segments treated with TA and TAMD were tested. The effect of TAMD treatment on resistance to proteolytic degradation was evaluated by measuring biomechanical properties of TAMD stabilized and non-stabilized SIS specimens after incubation in collagenase solution. It was shown that treatment with TA as well as with TAMD increased the strength of tubular SIS as well as their resistance to proteolytic biodegradation manifested by preservation of biomechanical properties after collagenase treatment. Transmission electron microscopy demonstrated that treatment with TAMD increased the periodical pattern typical of collagen fiber ultrastructure as a result of the "mordant" effect. The possible collagen cross-linking effect of TAMD on SIS was investigated by differential scanning calorimetry (DSC). The treatment with TAMD induced a small, but detectable cross-linking effect, suggesting that TAMD do not establish extensive covalent cross links within the extracellular matrix but rather interact with collagen, thus rendering SIS scaffolds more resistant to proteolytic degradation.

Original languageEnglish
Pages (from-to)745-751
Number of pages7
Issue number5
Publication statusPublished - Feb 2006


  • Acellular scaffold
  • Biomechanical properties
  • Dendrimer
  • Nanomordant
  • Tannic acid
  • Vascular prosthesis

Field of Science*

  • 1.6 Biological sciences
  • 2.4 Chemical engineering
  • 2.5 Materials engineering

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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